牛源金黄色葡萄球菌FnbpA-A基因克隆及抗原表位预测

Abstract

Abstract: A pair of specific primers was designed on A domain of FnbpA gene sequence of S.aureus published in GeneBank. Then FnbpA-A gene was cloned and sequenced, its protein second structure was analyzed and antigenic epitope was predicted. The sequencing results showed that FnbpA-A gene was 1645 bp in size, encoding 548 amino acids, the homology of coding amino acids between this strain and CIG1835 strain was 100%. Protein secondary structure analysis showed that FnbpA-A protein had alpha helixes 13.69%, β sheet 30.84%, β turn 11.86% and random coil 43.61%. B-cell epitopes were most likely localized in its N-teminal 31 to 46, 54 to 69, 142 to 157, 303 to 318, 324 to 339, 450 to 465, 491 to 506, 533 to 548 sites and T-cell epitopes had the high probability in the regions of 353 to 361 and 245 to 253. To sum up, FnbpA-A could be used as a candidate gene of S.aureus genetic engineering subunit vaccine.

Key words: font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Sfont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">.aureus; FnbpAfont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">-font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Afont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US"> gene; cloning; antigen epitopes prediction

HE Yan，HAO Yong-qing. Cloning and Antigen Epitopes Prediction of Fibronection-binding Protein A Gene A Domain of Bovine Staphylococcus aureus[J]. .

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Cloning and Antigen Epitopes Prediction of Fibronection-binding Protein A Gene A Domain of Bovine Staphylococcus aureus

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