猪圆环病毒2型TaqMan荧光定量PCR检测方法的建立

Abstract

Abstract: The specific primers and probe were designed according to the nucleotide sequence of porcine circovirus type 2 (PCV2) available in GenBank，CAP gene was amplified by PCR, cloned into the pMD18-T vector and screened positive plasmid standards.By optimization of reaction conditions，we established a TaqMan Real-time PCR method for detection of PCV2. The results indicated that the method was specific， and swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus (PRRSV), pig pseudorabies virus (PRV) and classical swine fever virus detection results were negative. The detection limit of the assay was 4.53×10² copies/μL of plasmid DNA，100 times higher than that of the routine PCR. The standard curve displayed a linear range from 10² to 10⁹ copies/μL and it had a good reproducibility.

Key words: porcine circovirus type 2; Real-time PCR; TaqMan probe

GUO Hui-juan1，2,LI Xiu-li2,ZHANG Guo-wei2,YAN Ming-hua2,LI Hai-hua2,LI Fu-qiang2. Establishment of TaqMan Real-time PCR for Detection of Porcine Circovirus Type 2[J]. .

References

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Establishment of TaqMan Real-time PCR for Detection of Porcine Circovirus Type 2

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