丙戊酸处理对猪手工克隆重构胚体外发育潜能的影响

Abstract

Abstract: To explore the effects of histone deaceylase inhibitors valproic acid (VPA) affecting the development potency of handmade cloned (HMC) embryos, porcine HMC reconstructed embryos were cultured for 24 h with different concentration (0，25，50，75 and 100 nmol/L) of VPA, and the blastocyst development rate, inner cell mass (ICM) cells number and histone acetylation level were compared. The results showed that reconstructed embryos were cultured in PZM-3 containing 50， 75 nmol/L VPA for 24 h, the frequency of the rate of cleavage and blastocyst formation were significantly higher in embryos treated with VPA than 0, 25, 100 nmol/L VPA treatments （P＜0.05）. In addition, 50 nmol/L VPA treatment significantly increased the number of inner cell mass (ICM) cells compared with the other groups (P＜0.05). The AcH3K14 level in embryos treated with VPA was higher than the control group and parthenogenetic activation group. In conclusion, epigenetic modification VPA could enhances the rate of cleavage, blastocyst formation and the ICM cells number of porcine HMC reconstructed embryos, which mean improve the developmental potency of HMC embryos.

Key words: porcine; handmade cloning; valproic acid (VPA); developmental potency

XIE Bing-kun, LV Ling-yan, LU Xing-rong，CUI Kui-qing, SUN Jun-ming, QIN Zhao-xian,SHI De-shun, LIU Qing-you. Effect of Valproic Acid on in vitro Developmental Competence of Handmade Cloned Pig Reconstructed Embryos[J]. .

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Effect of Valproic Acid on in vitro Developmental Competence of Handmade Cloned Pig Reconstructed Embryos

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