牛病毒性腹泻—黏膜病病毒C24V株E0截短基因密码子优化及其在卡介苗中的表达

Abstract

Abstract: The protein encoded by E₀ gene is one of the major protective antigens of bovine viral diarrhea-mucosal disease virus (BVDV). E₀ gene is a candidate for developing genetic engineering vaccine of BVDV and it could not express in BCG, it has been shown that E₀ protein has RNase activity, and has a plurality of rare codons according to analyze the codon usage frequency of BCG. By truncating the gene and optimizing its codons, E₀ gene expressed in BCG successfully and its antigenic activity was detected, which laid the foundation for building bivalent genetic engineering vaccine to prevent tuberculosis and bovine viral diarrhea-mucosal disease at the same time.

Key words: bovine viral diarrhea-mucosal disease virus; E₀ gene; codon; recombinant BCG

ZHANG Yun, HAO Jun-wei, LIU Hong-na, WANG Wen-yu, YANG Yu-hang, SHI Kun, LI Jian-ming, DU Rui. Codon Optimization and Expression in BCG of E₀ Truncated Gene from C₂₄V Strain of Bovine Viral Diarrhea-Mucosal Disease Virus[J]. .

References

1 白霞, 李润成, 余兴龙, 等. 猪瘟病毒 E^rns基因 RNase 酶活性位点的定点突变对其原核表达的影响［J］. 湖南农业大学学报, 2008, 34(5): 568～571.
2 刘晶, 于浩, 杨润军, 等. 牛乳蛋白的遗传密码子使用频率分析［J］. 中国兽医学报, 2011, 31(2): 267～271.
3 刘礼兵, 刘云, 何华庆, 等. qa_3稀有密码子和mRNA结构改造及其在大肠杆菌中的高效表达［J］. 生物工程学报, 2006, 22(2): 198～203.
4 刘德辉, 黄毓茂, 徐蕙, 等. 蝉抗菌肽基因密码子优化及其毕赤酵母表达载体构建［J］. 中国畜牧兽医, 2009, 36(10): 44～46.
5 严伟行. 牛病毒性腹泻病毒E₀基因的原核表达与活性检测［J］. 中国畜牧兽医, 2013, 40(1): 54～56.
6 吴立君, 师东方, 王丹娜, 等. 牛病毒性腹泻病毒E^ns基因的真核表达及抗原性检测［J］. 中国兽医科学, 2006, 36(6): 439～443.
7 沈冰蕾, 刘博洋, 杨润军, 等. 人溶菌酶基因的密码子优化及生物信息学初步分析［J］. 中国兽医学报, 2012, 32(9): 1392～1395.
8 罗丹丹, 李兴玉, 叶勇刚, 等. 猪细小病毒NS1基因密码子偏爱性分析［J］. 中国动物传染病学报, 2012, 20(5): 60～67.
9 郜玉刚. 鹿源BVDV分离鉴定、E₀基因的克隆与表达及免疫原性研究［D］. 长春:吉林农业大学, 2005.
10 崔保安, 朱沙, 陈圣先. 牛病毒性腹泻病毒蛋白特性的研究进展［J］. 中国畜牧兽医, 2003, 30(2): 32～34.
11 Collett M S, Larson R, Gold C, et al. Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus［J］. Virol, 1988, 165: 191～199.
12 Collett M S, Wiskerchen M, Welniak E, et al. Bovine viral diarrhea virus genomic organization［J］. Arch Viro1, 1991, Supp13: 19～27.
13 Larsson B, Terpstra C. Bovine viral diarrhea virus infections in piglets from sows vaccinated against swine fever with contaminated vaccine［J］. Res Vet Sci, 1995, 58: 186～189.
14 Rumenapf T, Unger G, Strauss J H, et al. Processing of the envelop glycoproteins of the pestiviruses［J］. Ｖirol, 1993, 67: 3288～3294.
15 Schneider R, Unger G, Stark R, et al. Identification of a structural glycoprotein of an RNA virus as a ribonuclease［J］. Science, 1993, 261: 1169～1171.

[1]	SUN Shouhu, CHEN Zhifei, GUO Zhixuan, YU Ting, WANG Xiuwu, LI Songbei, WEI Wenkang, HE Dongsheng. Study on Codon Usage Bias of N gene of Three Porcine Coronaviruses and Their Host Adaptive Mechanism [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2403-2413.
[2]	ZHAO Yu, ZHAO Ruli, GAO Yuan. Analysis of Codon Preference in Camelus ferus Genome [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 429-439.
[3]	WANG Jing, ZHU Zhe, ZHANG Peng, BI Yanzhen. Site-directed Mutagenesis of Porcine Myostatin Gene Using Single Base Editor [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2880-2887.
[4]	MA Jianqing, YANG Qingfang, SONG Zhanfeng, JIA Dingding, WU Dongwei, MO Xiufang, XU Zengnian, YANG Ke, WU Zhanyong, JI Ying, ZHOU Rongyan. Analysis of Codon Usage Bias of VDR Gene Related to Bone Metabolism in Ovis aries [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2888-2898.
[5]	LIU Xinrui, WANG Jiabo, CHAI Zhixin, WU Zhijuan, YIXI Kangzhu, ZHONG Jincheng. Comparison of X Chromosome Gene Coding Regions and Analysis of Codon Bias in Bos grunniens, Bos taurus and Bubalus bubalis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2043-2055.
[6]	HUANG Shuqin, HUANG Wenjing, LI Wenfeng, HONG Rui, HUANG Shujian, CHEN Yong. A Construction of Recombinant BCG Expressing Ag85 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 590-596.
[7]	DAI Kaiyu, HUANG Yanjie, WU Lisi, BAO Wenbin, WU Shenglong. Analysis and Optimization of Codon Usage Bias of Porcine FUT2 Gene [J]. , 2018, 45(7): 1863-1872.
[8]	GAO Hua, ZHAI Xiaoxin, JIANG Ping, GAN Hui, ZHANG Zonghui, XU Chongbo, GAO Fengshan. Construction and Expression of Tetramer Precursor Chain of SLA-3-HB01 Gene in Hebao Pig [J]. , 2018, 45(10): 2691-2699.
[9]	LI Wenfeng, MEI Minmin, HUANG Xingguo, HUANG Wenjing, LI Xiaowen, Saeed EL-ASHRAM, HUANG Shujian, LI Zhili. Optimization and Prokaryotic Expression of the σB Protein Gene Codons of the New-type Duck Reovirus [J]. , 2018, 45(10): 2700-2706.
[10]	FAN Xin-yang, OUYANG Yi-na, WANG Peng-wu, ZHANG Yong-yun, TENG Xiao-hong, MIAO Yong-wang. Analysis of Codon Usage Bias of Leptin Receptor Gene in Buffalo [J]. , 2017, 44(4): 1069-1078.
[11]	ZHANG Xiao-qing, TONG Xiao-ming, WEN Wei-ye. Effects of Compound Codonopsis Oral Liquids on Immune Molecules with Immunological Stress in Layer Chickens [J]. , 2016, 43(8): 2066-2071.
[12]	LU Guo-min, ZHANG Can, ZOU Ling, LIU Wen-hua, WEN Jian-xin, REN Hui-ying. Analysis of Codon Bias on Lysozyme Gene of Phage vB_EcoM-Bp7 [J]. , 2015, 42(9): 2233-2239.
[13]	BAO Zhen-zhong, LEI Cheng-hong, CAI Yuan-qing, GAO Dou, BIAN Sai-sai, GE Ting. Isolation,Identification and Biological Characteristics Analysis of Bovine Viral Diarrhoea-mucosal Disease Virus [J]. , 2015, 42(9): 2391-2398.
[14]	ZHANG Tao, WANG Wen-hao, ZHANG Gen-xi, WANG Jin-yu, FAN Qing-can, HAN Kun-peng, TANG Ying, WANG Yong-juan. Characterization of Codon Usage of GnRHR Gene in Jinghai Yellow Chicken [J]. , 2014, 41(12): 7-15.
[15]	LUO Dan-dan, CAO Ye, XIE Jing, ZHAO Su-jun, LI Jiang-ling, LIAO Dang-jin, YE Yong-gang, LI Xing-yu. Codon Preference Analysis of E2 Gene of Classical Swine Fever Virus [J]. , 2014, 41(12): 50-55.

Codon Optimization and Expression in BCG of E₀ Truncated Gene from C₂₄V Strain of Bovine Viral Diarrhea-Mucosal Disease Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Codon Optimization and Expression in BCG of E0 Truncated Gene from C24V Strain of Bovine Viral Diarrhea-Mucosal Disease Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Codon Optimization and Expression in BCG of E₀ Truncated Gene from C₂₄V Strain of Bovine Viral Diarrhea-Mucosal Disease Virus