猪流行性腹泻病毒研究进展及疫苗应用前景

Abstract

Abstract: In recent years, the epidemic intensity of porcine epidemic diarrhea virus (PEDV) have continuously enlarged,causing a huge economic losses in pig industry. This paper summarizes the research progress on the isolation and identification,variation, molecular biology of PEDV.This paper based on the current immunity results ot the PEDV vaccines, the research and development direction of PEDV production, with a view to the new, more useful product of PEDV.

Key words: PEDV; vaccine; molecular biology; epidemiology

CHEN Shen-miao，NIU Cheng-ming， HE Fu-qing，WU Xue-li，MA Jing-xia. Research Progress and Vaccine Prospect of Porcine Epidemic Diarrhea Virus[J]. .

References

1 于晓龙.猪流行性腹泻病毒和猪传染性胃肠炎病毒混合感染的调查［J］.安徽农业通报，2007,13（14）：156～157.
2 于强, 王殿瀛, 宣华, 等. 应用ELISA间接法检测猪流行性腹泻抗体的研究［J］. 中国兽医学报， 1987(2): 149～154.
3 卫龙兴，成建忠．上海规模化猪场猪病毒性腹泻的流行病学调查［J］.畜禽业，2010，259（11）：60～61.
4 王文梅, 许丽, 王秋菊. 乳酸菌对动物肠道的黏附作用研究进展［J］. 东北农业大学学报, 2009, 40(12): 120～124.
5 田小艳，孙华，邓雨修，等.3种猪病毒性腹泻感染的多重PCR检测［A］.中国畜牧兽医学会家畜传染病学分会第七届全国会员代表大会暨第十三次学术研讨会论文集（下册）［C］.2009.
6 李品齐, 丁敦志, 刘万钧. 猪流行性腹泻病毒在Vero细胞上的适应性试验［J］. 中国兽医杂志, 1997, 23(9): 24.
7 庄金秋, 王金良, 梅建国, 等. 猪流行性腹泻病毒SDbz株的分离与鉴定［J］. 动物医学进展, 2013, 34(2): 122～126.
8 刘云波, 赵洪翠, 王志成, 等, 猪病毒性腹泻分子流行病学调查［J］. 中国畜牧兽医, 2013, 40(2): 204～207.
9 刘孝珍, 陈建飞, 时洪艳, 等. 2011年猪流行性腹泻病毒的遗传变异分析［J］. 中国预防兽医学报, 2012, 34(3): 180～183.
10 刘随新, 石达, 陈建飞, 等. 猪流行性腹泻病毒N蛋白核仁定位信号对宿主细胞周期的影响［J］. 中国预防兽医学报, 2013, 35(3): 173～176.
11 肖少波, 陈焕春, 方六荣, 等. 伪狂犬病毒gD基因在转基因烟草中的表达［J］. 中国生物化学与分子生物学报, 2002, 18(4): 465～468.
12 肖贤, 陈备娟, 张风时, 等. 上海地区新发猪流行性腹泻病毒的鉴定及分析［J］. 上海交通大学学报，2013, 31(1): 61～66.
13 库旭钢, 刘云波, 凌泽熹，等. 猪病毒性腹泻的流行病学调查及分析［J］. 养猪，2013, 5: 101～102.
14 杨群, 何孔旺, 陆承平. 应用多重RT-PCR方法检测158例猪粪样中的两种冠状病毒［J］. 中国预防兽医学报, 2006, 4: 431～435.
15 郑逢梅, 霍金耀, 赵军, 等. 2010—2012年华中地区猪流行性腹泻病毒分子特征和遗传进化分析［J］. 病毒学报, 2013, 29(2): 197～205.
16 胡桂学，刁鹏祥，陈中秋，等.PEDV COE基因重组乳酸仔猪和妊娠母猪免疫研究［A］.中国畜牧兽医学会家畜传染病学分会第七届全国会员代表大会暨第十三次学术研讨会论文集（下册）［C］.2009.
17 修金生, 周伦江, 陈如敬, 等. 猪流行性腹泻病毒SYBRⅠ实时荧光定量RT-PCR检测方法的建立［J］. 中国兽医科学, 2012, 42(2): 160～165.
18 韩蓉, 时晓丽, 赵攀登, 等. 猪流行性腹泻病毒间接ELISA 抗体检测方法建立与应用［J］. 畜牧与兽医, 2013, 45(2): 1～6.
19 蒋梅, 王印, 杨泽晓, 等. 猪流行性腹泻病毒的分离鉴定及其M蛋白截段基因编码氨基酸的生物学分析［J］. 中国兽医学报, 2013, 33(2): 195～200.
20 董浩, 王莹. PEDV COE基因重组乳酸菌在小鼠体内定植规律研究［J］. 中国兽药杂志, 2011, 45(7): 6～9.
21 Awram P, Gardner R C, Forster R L, et al. The potential of plant viral vectors and transgenic plants for subunit vaccine production［J］. Adv Virus Res, 2002, 58: 81～124.
22 Bae J L, Lee J G, Kang T J, et al. Induction of antigen specific systemic and mucosal mimune responses by feeding animals stransgenic plants expressing the antigen［J］. Vaccine, 2003, 21: 4052～4058.
23 Calvo E, Escors D, Lopez J A, et al. Phosphorylation and subcellular localization of transmissible gastroenteritis virus nucleocapsid protein in infected cells［J］. J Gen Virol, 2005, 86(Pt8): 2255～2267.
24 Chen H Y, Wurm T, Britton P, et al. Interaction of the coronavirus nucleoprotein with nucleolar antigens and the host cell［J］. J Virol, 2002, 76: 5233～5250.
25 Cruz D J, Shin H J. Application of a focus formation assay for detection and titration of porcine epidemic diarrhea virus［J］. J Virol Methods，2007，145: 56～61.
26 Fraley R T, Rogers S G, Horsch R B, et al. Expression of bacterial genes in plant cells［J］. Proc N atl Acad Sci USA, 1983, 80(15): 4803～4807.
27 Gleba Y, Klimyuk V, Marillonnet S. Magnifection——A new platform for expressing recombinant vaccines in plants［J］. Vaccine, 2005, 23(17～18): 2042～2048.
28 Jinghui F, Yijing L. Cloning and sequence analysis of the M gene of porcine epidemic diarrhea virus LJB/03［J］. Virus Genes, 2005, 30（1）: 69～73.
29 Hou X L, Yu L Y, Lin J Z. Development and evaluation of enzyme-linked immunosorbent assay based on recombinant nucleocapsid protein for deteetion of porcine epidemic diarrhea (PEDV) antibodies［J］. Veterinary Microbiology, 2007, 123(1～3): 86～92.
30 Kadoi K, Sugiok H, Satoh T, et al. The propagation of a porcine epidemic diarrhea virus in swine cell lines［J］.New Microbial, 2002, 25: 285～290.
31 Knuchel M, Ackermann M, Muler H K, et al. An ELISA for detection of antibodies against porcine epidemic diarrhea virus (PEDV) based on the specific solubility of the viral surface glycoprotein［J］.Vet Micobiol, 1992, 32: 117～134.
32 Kolaskar A, Kulkarni-Kale U. Prediction of three-dimensional structure and mapping of conformational epitopes of envelope glycoprotein of Japanese encephalitis virus［J］. Virology, 1999, 261(1): 31～42.
33 Kwon H M, Saif L J, Jackwood D J. Field isolates of transmissible gastroenteritis virus differ at the molecular level from the Miller and Purdue virulent and attenuated strains and from porcine respiratory coronacirus［J］.J Vet Med Sci, 1998, 60(5): 589～597.
34 Laude H, Gelfi J, Lavenant L, et al. Single aminoacid changes in the viral glycoprotein M affect induction of alpha interferon by the coronavirus transmissible gastroenteritis virus［J］. J Virol, 1992, 66(2): 743～749.
35 Lee D K, Park C K, Kim S H, et al. Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea. Virus Res，2010，149（2）: 175～182.
36 Park S J, Moon H J, Yang J S, et al. Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea［J］. Virus Genes, 2007, 35: 321～332.
37 Paturi G, Phi llips M, Jones M, et al. Immunce enhancing effects of Lactobacillus acid ophilus LAFT IL10 and Lactobacillus paracasei LAFT IL26 in mice［J］. International Journal of Food Microbiology, 2007, 115(7): 115～118.
38 Pensaert M B, Debouck P. A new coronavirus-like particle associated with diarrhea in swine［J］. Arch Virol, 1978, 58 (3): 243～247.
39 Rodák L, Valícek L, Smíd B, et al. An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces［J］. Vet Microbiol, 2005, 105(1): 9～17.
40 Song D S, Yang J S, Oh J S, et al. Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF3［J］. Vaccine, 2003, 21: 1833～1842.
41 Sozzi E, Luppi A, Lelli D, et al. Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus［J］. Res Vet Sci ,2010, 88（1）: 166～168.
42 Sueyoshi M, Tsuda T, Yamazaki K, et al. An immunohistochemical investigation of porcine epidemic diarrhea［J］. J CompPathol, 1995, 113(1): 59～67.
43 Sun D, Feng L, Shi H, et al. Identification of two novel B cell epitopes on porcine epidemic diarrheavirus spike protein［J］. Vet Microbiol, 2008, 131: 73～81.
44 Sun R Q, Cai R J, Chen Y Q, et al. Outbreak of porcine epidemic diarrhea in suckling piglets, China［J］.Emerging Infectious Diseases, 2012, 18(1): 161～162.
45 Surjit M, Lal S K. The SARS-CoV nucleocapside protein: A protein with multifarious activities［J］. Infect Genet Evol, 2008, 8(4): 397～405.
46 Wurm T, Chen H Y, Hodgson T, et al. Localization to the nucleolus is a common feature of coronavirus nucleoproteins and the protein may disrupt host cell division ［J］. J Virol, 2001, 75（19）:9345～9356.
47 Yoo D, Wootton S K, Li G, et al. Colocalization and interaction of the porcine arterivirus nucleocapsid protein with the small nucleolar RNA associated protein fibrillarin［J］. J Virol, 2003, 77(22): 12173～12183.

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Research Progress and Vaccine Prospect of Porcine Epidemic Diarrhea Virus

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