Abstract: In order to investigate the effect of glucose on oxidative stress in cultured goose primary hepatocytes, partial gene sequences of GPX1 and SOD1 genes were cloned, and the goose primary hepatocytes were treated with different concentrations（0（control group）, 5, 25 and 35 mmol/L） of glucose for 48 h, and then enzyme activities of GPX and SOD and mRNA levels of GPX1 and SOD1 were examined. The results showed that goose GPX1 gene sequence had the highly similarity with Zebra Finch, and SOD1 had the highly similarity with Red Jungle fowl. Compared with the control group, 5 mmol/L glucose had no significant effect on GPX enzyme activity （P>0.05）, but 25 and 35 mmol/L glucose could significantly decrease GPX enzyme activity (P＜0.05).5 mmol/L glucose could significantly increase SOD enzyme activity （P＜0.05）, but 25 and 35 mmol/L glucose had no significant effects on SOD enzyme activity（P＞0.05）.35 mmol/L glucose could significantly increase mRNA expression of GPX1 and SOD1 genes （P＜0.05）, but 5 and 25 mmol/L glucose had no significant effects on the two genes（P＞0.05）.In conclusion, the high concentration glucose （35 mmol/L） could induce intracellular oxidative stress in goose primary hepatocytes.

Key words: goose; glucose; glutathione peroxidase enzyme; superoxide dismutase enzyme; oxidative stress