猪源植物乳杆菌的诱变选育及其对小鼠免疫性能的影响

Abstract

Abstract: To get a Lactobacillus plantarum strain with good acid resistance, cholate tolerance, and in vitro adhesion rate and study its effects on immune function of mice. After screening Lactobacillus plantarum by acid resistance and cholate tolerance after UV mutagenesis, we got BL-15 strain, which was cultured, freeze-dried, dissolved and administered to BALB/c mice orally (gastric perfusion) by three times. Blood, spleen and excrement were collected to detect changes in immune function index.One Lactobacillus plantarum strain BL-15 was selected from 17 mutant strains, with higher acid resistance, cholate tolerance and in vitro adhesion rate. The experimental data of animal tests indicated that IL-2 level and spleen index of mice at early growth stage had been increased. Furthermore, the mice treated with BL-15 as the immunity enhancer revealed higher level of SIgA in intestinal contents compared with control (P<0.05). The results showed that Lactobacillus plantarum strain BL-15 had been proved to be able to elevate immune response and enhance immune function.

Key words: Lactobacillus plantarum; mutation breeding; mice; immune function

CHENG Fu-liang，WANG Chun-feng，NIE Zhao-jing，JIANG Yan-ping，WANG LI-rong，LIU Yang-qing，GU Wei. Mutation Breeding of Porcine Lactobacillus plantarum and its Effects on Immune Function of Mice[J]. .

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Mutation Breeding of Porcine Lactobacillus plantarum and its Effects on Immune Function of Mice

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