Abstract: In order to provide a more dependent and fine tool for porcine immunology research, we developed a serial of pig specific NF-κB reporter system. Firstly, 6 reporter vectors were constructed by using 6 NF-κB recognition sites. Through transfection of porcine iliac endothelial cell line (PIEC), porcine alveolar macrophages cell line 3D4/21 and porcine primary fetal fibroblasts(CPEF), the relative luciferase expression was detected. These 6 reporter vectors, constructed in this study, could effectively report the expression of p65 subunit of porcine NF-κB, and the upregulation fold-changes were all higher than that of pNF-κB-Luc. The luciferase activities of reporter vectors A to F displayed regular difference among each other, of which reporter vector C showed the highest activities, while reporter vector F was the lowest. Compared to the commercial available NF-κB reporter vetor, those all 6 reporter vectors showed higher specificity of transcription activities. So our vector series could be used as NF-κB reporter vectors for the porcine species.

Key words: NF-κB reporter vector; immunity; porcine; specificity