重组鸡α-干扰素的表达及其生物活性的初步研究

Abstract

Abstract: In order to obtain the recombinant chicken interferon-α (ChIFN-α) and study its biological activity, the recombinant E.coli was induced to express ChIFN-α. After ChIFN-α inclusions were collected for renaturation and purification, Western blotting test for the recombinant ChIFN-α was done. By measuring the EID₅₀ of chicken embryos infected with avian influenza virus (AIV) H9 subtype, Newcastle disease virus (NDV) and infectious bronchitis virus (IBV), we investigated the antiviral activity of the recombinant ChIFN-α and determined its antiviral potency by cytopathic effect inhibition assay. The specific bands were found as expected in Western blotting test of renaturated ChIFN-α. In addition, the ChIFN-α showed significant antiviral activity against AIV H9, NDV and IBV, and antiviral potency of the interferon was about 1.5×2¹⁰ U according to the test on CEF/VSV cell line. High-concentration ChIFN-α showed cytotoxicity. The recombinant ChIFN-α had immunogenicity and antigenicity. And it could inhibit or kill viruses in chicken embryos including AIV H9, NDV and IBV, which showed broad spectrum and high antiviral activity. The study would promote our research and development of antiviral products in the future.

Key words: chicken interferon-α (ChIFN-α); expression; biological activity

CLC Number:

Q789

CHENG Fu-liang, NIE Zhao-jing, CHEN Tian-tian, LIU Yang-qing, FANG dong, CUI Zhao-lian, GU Wei, WANG Chun-feng. Preliminary Study on Expression of Recombinant Chicken IFN-α and its Biological Activity[J]. , 2014, 41(11): 35-39.

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Preliminary Study on Expression of Recombinant Chicken IFN-α and its Biological Activity

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