猪繁殖与呼吸综合征病毒分离株GP5蛋白ORF5基因的克隆与序列分析

Abstract

Abstract: In order to reveal the Guangdong region of porcine reproductive and respiratory syndrome (PRRS) epidemic law, 5 strains of swine clinical samples amplified from Guangdong region of PRRSV GP5 genes of the isolates with the method of RT-PCR, cloned, sequenced and domestic and foreign strains corresponding gene nucleotide sequence comparison in this study.The results showed that PRRSV-2011-GD2, PRRSV-2011-GD3, PRRSV-2013-GD1, PRRSV-2013-GD2 and PRRSV-2013-GD3 strains with the American type reference strains (ATCC VR-2332 U87392) nucleotide sequence homology were 88.8%, 99.5%, 88.7%, 88.7% and 83.5%,respectively. The phylogenetic tree analysis indicated that PRRSV-2011-GD2, PRRSV-2011-GD3, PRRSV-2013-GD1, PRRSV-2013-GD2 and PRRSV-2013-GD3 strains belonged to the American.The results provided the basis for a better understanding of the molecular epidemiology of PRRSV features and antigenic variation law, also laid the foundation for the study of gene engineering vaccine.

Key words: porcine reproductive and respiratory syndrome; GP5 protein; sequence analysis

CLC Number:

S854.4⁺3

WU Xiao-wei, LIU Xiao-hui, YANG Qian, XIONG Rui, GUO Feng-liu, GUO Xiao-feng, YANG You-tian, CHEN Ru. Cloning and Sequence Analysis of GP5 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains[J]. , 2014, 41(11): 40-43.

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Cloning and Sequence Analysis of GP5 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains

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