上海市零售禽肉制品和活禽中沙门氏菌血清型与耐药性研究

Abstract

Abstract: The purpose of this study was to understand the pollution condition,popular serotype and drug resistance of Salmonella in farmer's market and supermarket, in Shanghai. A total of 240 chicken samples and 80 chickens' cloaca swabs were obtained from market in 2012. Among them, 70 (21.9%) were positive for Salmonella. Of the 11 serotypes in 70 Salmonella isolates, S.enteritidis (47.1%), S.indiana (17.1%), S.typhimurium (8.6%) and S.derby (8.6%) were prevalent.Drug susceptibility test was conducted among the Salmonella isolates using the Kirby-Bauer disk diffusion method. The highest level of resistance was found for nalidixie acid (75.7%), followed by sulfisoxazole (60.0%), streptomycin (60.0%) and ampicillin (51.4%). No resistance to imipenem was observed in any isolate examined.The isolated chicken Salmonella strains had severe resistance to most drugs. 40 strains of Salmonella were millti-drug resistant,the most resistart strain was resistant to 14 drugs.This study had important practical significance for prevention and control and rational drug use of salmonellosis.

Key words: Salmonella; serotype; drug resistance

CLC Number:

S852.61⁺4

SHEN Hai-yan, GUO Hui-xia, XU Xue-bin, ZHOU Heng, LIU Zhi-cheng, ZHANG Chun-hong. Study on Serotype and Drug Resistance of Salmonella from Retail Poultry Products and Live Poultry in Shanghai[J]. , 2014, 41(11): 263-266.

References

1 王茂起,冉陆,王竹天,等. 2001年中国食源性致病菌及其耐药性主动监测研究[J]. 卫生研究,2004,33(1):49～54.
2 王晓泉,焦新安,刘晓文,等. 江苏部分地区食源性和人源性沙门氏菌的多重耐药性研究[J].微生物报,2007,47(2):221～227.
3 石晓路,刘小立,黄薇,等. 脉冲场凝胶电泳在沙门菌食物中毒溯源中的应用研究[J].疾病控制杂志,2007,11(5):502～504.
4 杨筱薇,旷代,潘海建,等. 上海市禽源阿贡纳沙门氏菌耐药性及脉冲场凝胶电泳分型研究[J]. 中国畜牧兽医,2014,41(2):213～217.
5 杨德胜,张险朋,黄炳炽,等. 动物产品沙门氏菌污染情况调查[J]. 中国畜牧兽医,2010,37(10):202～203.
6 张秀丽,廖兴广,郝宗宇,等. 2006～2007年河南省生肉食品中沙门菌的主动监测及其DNA指纹图谱库的建立[J]. 中国卫生检验杂志,2009,19(7):1545～1548.
7 张玮,李郁,姚健,等. 健康猪直肠粪便中沙门菌Ⅰ类整合子与耐药基因的检测[J]. 中国微生态学杂志,2010,22(7):594～597.
8 张茂棠,林琳,刘渠,等. 深圳市生鸡肉中沙门菌的耐药性研究[J]. 现代预防医学,2005,32(7):732～733.
9 周佳,刘书亮,侯小刚,等. 四川省动物性食品源沙门氏菌的耐药性监测与分析[J]. 中国畜牧兽医,2011,38(3):188～191.
10 赵玉林. 鸡源多重耐药沙门氏菌Ⅰ类整合子与耐药基因特征研究[D]. 杨凌:西北农林科技大学,2012.
11 焦新安,涂长春,黄金林,等. 我国食源性人兽共患细菌病流行现状及其防控对策 [J].中国家禽,2009,31(19):4～11.
12 Angulo F J, Baker N L, Olsen S J, et al. Antimicrobial us in agriculture: Controlling the transfer of antimicrobial resistance to humans[J].Seminars in Pediatric Infectious Diseases,2004,15(2): 78～85.
13 CLSL. M100-S19 performance standards for antimicrobial susceptibility testing[S]. Wayne,Clinical and Laboratory Standards Institute, 2009.
14 Francois X W, Francoise G. Multidrug resistance in Salmonella enterica serotype typhimurium from humans in France[J]. J Clin in Microbiol, 2006, 44(3):700～708.
15 Yang H, Chen S, White D G, et al. Characterization of multiple antimicrobial resistant Escherichia coli isolates from diseased chickens and swine in China[J]. J Clin Microbiol, 2004,42(8):3483～3489.
16 Zhang J, Jin H, Hu J, et al. Serovars and antimicrobial resistance of non-typhoidal Salmonella from human patients in Shanghai, China, 2006—2010[J]. Epidemiol Infect, 2014, 142(4):826～832.

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Study on Serotype and Drug Resistance of Salmonella from Retail Poultry Products and Live Poultry in Shanghai

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