水貂酪氨酸酶基因外显子1的鉴定及序列变异分析

Abstract

Abstract: One pair of special primer was designed according to the conservative region of tyrosinase (TRY) gene in Mustela putorius furo, Ailuropoda melanoleuca, Canis lups familiaris and Felis catus deposited in GenBank database. The partial sequence of exon 1 from Neovison vison(Black), Neovison vison(Regal White) and Neovison vison (Palomino) mink were obtained by PCR amplification and cloning sequencing. The results showed that the length of TYR gene in mink with three kinds of coat color was 796 bp, comprising partial exon 1(795 bp) and 5'UTR (1 bp). Five singleton variable sites were detected at position 34 (A/G), 138 (T/A), 248(T/C), 545(A/G) and 765(C/A), including four missense mutations and one frameshift mutation. The accession numbers were KJ198847(Black), KJ658343(Regal White) and KJ152769 (Palomino), respectively. The nucleotide similarity between mink and ferret, giant panda, dog, cat, rabbit, pig, alpaca, goat, sheep, cattle, human, rhesus monkey, chimpanzee, chicken and japanese quail was 72.8% to 98.6%, and the amino acid similarity was 75.0% to 98.1%. The phylogenetic tree constructed by TYR amino acid indicated that the evolution distance of mink was the most homogeneous to ferret beloning to Mustelidae. This study would lay the bioinformatics foundation for further investigating correlations between polymorphisms of TYR gene and coat color phenotype in mink.

Key words: mink; variation of coat color; tyrosinase gene; genetic evolution

CLC Number:

S813.3

SONG Xing-chao, XU Chao, YUE Zhi-gang, WANG Lei, CONG Bo, LIU Lin-ling, YANG Fu-he. Identification and Sequence Variation Analysis of TYR Gene Exon 1 in American Mink (Neovison vison)[J]. , 2014, 41(11): 205-212.

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Identification and Sequence Variation Analysis of TYR Gene Exon 1 in American Mink (Neovison vison)

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