贵州地方山羊促卵泡素受体基因部分外显子多态性研究

Abstract

Abstract: In this study, Guizhou White goat, Qianbei Ma goat, Guizhou Black goat from Guizhou native goat breeds were selected for testing subjects, the technology of DNA pools and the direct sequencing of PCR products were used to detect the single nucleotide polymorphism (SNP) of follicle stimulating hormone receptor (FSHR) gene in goat. Moreover, through bioinformatics software to analyze the impact of secondary structure of RNA. The results showed that three SNPs (C126T,C1246A and A1412C) were found, among these SNPs, C126T and C1246A lay in the exon 1 and exon 10, which were sense mutation and the A1412C lay in intron. Bioinformatics analysis that the two SNPs in the exon 1 and exon 10 lead to the alteration of secondary structure of mRNA.

Key words: follicle stimulating hormone receptor; SNPs; exon; goat

CLC Number:

S813.3

LONG Wei-hai, DING Mei, FENG Wen-wu, XU Hou-qiang, CHEN Wei, ZHANG Yong, CHEN Xiang. Study on the Polymorphism for Part of FSHR Gene Exon in Guizhou Native Goat[J]. , 2014, 41(11): 198-204.

References

1 王明亮, 高旭东, 陈鹏, 等. 牦牛FSHR基因部分序列多态性及其与繁殖性状的关联分析[J]. 兽类学报, 2012, 32(3):239～247.
2 何荆州. 荷斯坦奶牛FSHR基因5'侧翼和第10外显子多态性及其与超数排卵关系的研究[D]. 广西:广西大学,2008.
3 吴井生,王金玉.FSHR基因第10外显子多态性及其与小梅山猪产仔数的相关性[J]. 中国农业科学, 2012, 45 (13):2728～2736.
4 宋美玲, 马向明, 王建民. 波尔山羊FSHR 5'端序列的SNP多态性及超排效果分析[J]. 家畜生态学报, 2006, 27(4): 25～28.
5 陈洋, 陈娟, 杨永梅,等. 促卵泡素受体基因的研究概况[J]. 中国畜牧兽医, 2010, 37(2): 108～110.
6 陈琰, 徐仙, 刘春莲, 等. 不孕妇女FSHR基因多态性检测及卵巢功能与临床结局的研究[J]. 宁夏医学杂志, 2012, 34(9): 824～826.
7 雷雪芹, 陈宏, 袁志发, 等. 牛FSHR基因第10外显子单核苷酸多态性及其与双胎性状的关系[J]. 中国生物化学与分子生物学报,2004,20(1):34～37.
8 Balkan M, Gedik A, Akkoc H, et al. FSHR single nucleotide polymorphism frequencies in proven fathers and infertile men in Southeast Turkey[J]. Biomed Research International, 2010, 2010:640318.
9 Chu M X, Guo X H, Feng C J, et al. Polymorphism of 5' regulatory region of ovine FSHR gene and its association with litter size in Small Tail Han sheep[J]. Molecular Biology Reports, 2012, 39(4): 3721～3725.
10 de Castro F, Ruiz R, Montoro L, et al. Role of follicle-stimulating hormone receptor Ser680Asn polymorphism in the efficacy of follicle-stimulating hormone[J]. Fertility and Sterility, 2003, 80(3): 571～576.
11 Du J, Zhang W, Guo L, et al. Two FSHR variants, haplotypes and meta-analysis in Chinese women with premature ovarian failure and polycystic ovary syndrome[J]. Molecular Genetics and Metabolism, 2010, 100(3): 292～295.
12 Gromoll J, Pekel E, Nieschlag E. The structure and organization of the human follicle-stimulating hormone receptor (FSHR) gene[J]. Genomics, 1996, 35(2): 308～311.
13 Greb R R, Grieshaber K, Gromoll J, et al. A common single nucleotide polymorphism in exon 10 of the human follicle stimulating hormone receptor is a major determinant of length and hormonal dynamics of the menstrual cycle[J]. Journal of Clinical Endocrinology & Metabolism, 2005, 90(8): 4866～4872.
14 Heubner M, Riemann K, Otterbach F, et al. The haplotype of two FSHR polymorphisms in ovarian cancer——A potential role of ethnology in risk modification[J]. Gynecologic Oncology, 2009, 112(3): 486～489.
15 Kim S, Pyun J A, Cha D H, et al. Epistasis between FSHR and CYP19A1 polymorphisms is associated with premature ovarian failure[J]. Fertility and Sterility, 2011, 95(8): 2585～2588.
16 Li Y, Gu A, Yang H, et al. FSH receptor gene polymorphisms in fertile and infertile Han-Chinese males[J]. Clinica Chimica Acta, 2011, 412(11): 1048～1052.
17 Milazzotto M P, Rahal P, Nichi M, et al. New molecular variants of hypothalamus-pituitary-gonad axis genes and their association with early puberty phenotype in Bos taurus indicus (Nellore)[J]. Livestock Science, 2008, 114(2): 274～279.
18 Mohiyiddeen L, Newman W G, Cerra C, et al. A common Asn680Ser polymorphism in the follicle-stimulating hormone receptor gene is not associated with ovarian response to gonadotropin stimulation in patients undergoing in vitro fertilization[J]. Fertility and Sterility, 2013, 99(1): 149～155.
19 Rannikki A S, Zhang F P, Huhtaniemi I T. Ontogeny of follicle-stimulating hormone receptor gene expression in the rat testis and ovary[J]. Molecular and Cellular Endocrinology, 1995, 107(2): 199～208.
20 Sang L, Du Q Z, Yang W C, et al. Polymorphisms in follicle stimulation hormone receptor, inhibin alpha, inhibin bata A, and prolactin genes, and their association with sperm quality in Chinese Holstein bulls[J]. Animal Reproduction Science, 2011, 126(3): 151～156.
21 Yang W C, Li S J, Xie Y H, et al. Two novel SNPs of the type Ⅰ gonadotropin releasing hormone receptor gene and their associations with superovulation traits in Chinese Holstein cows[J]. Livestock Science, 2011, 136(2): 164～168.
22 Zerbetto I, Gromoll J, Luisi S, et al. Follicle-stimulating hormone receptor and DAZL gene polymorphisms do not affect the age of menopause[J]. Fertility and Sterility, 2008, 90(6): 2264～2268.

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Study on the Polymorphism for Part of FSHR Gene Exon in Guizhou Native Goat

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