亮甲酚蓝染色对牛卵母细胞体外成熟和凋亡的影响

Abstract

Abstract: The aim of this investigation was to study the effect of brilliant cresyl blue (BCB) staining on IVM apoptosis in cow, COCs without BCB staining was considered as control group, and COCs stained with BCB in 26 μmol/L for 90 min as treatment group before mature culture. Treated oocytes were divided into BCB^-(colourless cytoplasm) and BCB⁺(coloured cytoplasm). After IVM, to assess the developmental potential of the oocytes. The results showed that, the BCB⁺ oocytes had a significantly higher proportion of mature rate (80.92%) than control group (60.00%) and BCB^-oocytes (51.61%)(P<0.05);The BCB⁺ oocytes yielded a significantly lower apoptosis rate (6.50%) than that in control (28.21%) and BCB^- oocytes (39.06%)(P<0.05). These results suggested that the staining of cow cumulus-oocyte complexes with BCB before in vitro maturation could be used to select developmentally competent oocytes.

Key words: cow; oocytes; brilliant cresyl blue staining; IVM

CLC Number:

Q813.1

QIAO Li-min, QIAO Fu-qiang, YAO Hua, LEI Li-hui, WANG Jin-qiu, GUAN Wei-jun, MA Yue-hui. Effect of BCB Staining on the IVM Apoptosis of Cow Oocytes[J]. , 2014, 41(11): 171-174.

References

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Effect of BCB Staining on the IVM Apoptosis of Cow Oocytes

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