抗猪流行性腹泻病毒卵黄抗体的制备及应用

Abstract

Abstract: Laying hens were immunized with porcine epidemic diarrhea virus (PEDV) SC strains. Immunoglobin of yolk (IgY) from chick yolk was extracted and purified by water dilution-sodium sulfate method and water dilution-sodium sulfate-salting method after harvesting eggs and separating egg yolk. Treatment and security were determined with 3-day-old piglets without maternal antibodies. IgY was used in naturally infected farms to further validate the therapeutic effect. The experimental results showed that high level of specific antibodies were produced after the hens immunized with PEDV SC strains in second times. In the treatment trial,survival rate of infection and treatment gnoup piglets was 60%,the challenge control group survival rate was 20%. In the naturally infected farms,survival rate of treatment group piglet was also 60%,the control group was 10%. These results suggested that anti-PEDV IgY could treat piglets infected with PEDV.

Key words: porcine epidemic diarrhea virus (PEDV); laying hens; immunoglobin of yolk (IgY)

CLC Number:

Q813

LI Bing, ZHANG Xian-hao, PEI Zhang-fu, CHEN Rui-ai, HE Dong-sheng. Preparation and Application of Immunoglobin of Yolk against Porcine Epidemic Diarrhea Virus[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(10): 68-72.

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Preparation and Application of Immunoglobin of Yolk against Porcine Epidemic Diarrhea Virus

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