鸡主要组织相容性复合体Ⅰβ微球蛋白(β2m)基因的克隆及序列分析

Abstract

Abstract: In order to further understand molecular characteristics and immune mechanisms of chicken major histocompatibility complex class Ⅰ(MHCⅠ) β2m gene,the 15 sequence of chicken MHC β2m gene from three local breeds was cloned by RT-PCR. We compared these sequences with seven MHCⅠβ2m genes of human,mouse and other animals in the GenBank database. The results showed that the amino acid homology among chicken MHCⅠβ2m ranged from 98.0% to 100%,the most sequences were identical. Chicken MHCⅠβ2m shared a 60.6% amino acid homology with duck,the highest degree of homology indicated a close genetic relationship,and the grasscarp had the lowest homology,33.3%. It could be further confirmed by phylogenetic analysis. Moreover,alignment of 10 β2m sequences of mature protein,two cysteines were located in sites 24 and 79,and there was the "YXCXVXH" Ig-motif character between the sites 77 and 83 of chicken β2m. The results showed that MHCⅠβ2m of chicken and other species had the same basic unit of immune function,and they had the unique structural features.

Key words: chicken MHC Ⅰ; β2m gene; sequence analysis

CLC Number:

DAI Yin, ZHANG Dan-jun, HU Xiao-miao, ZHAO Rui-hong, HOU Hong-yan, PAN Xiao-cheng, ZHOU Xue-li, SHEN Xue-huai. Cloning and Sequence Analysis of MHC Ⅰ β2m Gene in Chicken[J]. , 2014, 41(10): 28-32.

References

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Cloning and Sequence Analysis of MHC Ⅰ β2m Gene in Chicken

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