猪葡萄球菌EXHC、SHETA基因的克隆、序列分析及蛋白结构预测

Abstract

Abstract: It was to analyze and predict the structural characteristics of EXHC and SHETA genes from Staphylococcus hyicus exfoliative toxins by bioinformatics. According to Staphylococcus hyicus exfoliative toxins EXHC and SHETA genes sequence reported in GenBank,two pairs of primers were designed to amplify EXHC and SHETA gene fragments,respectively. The fragment size of EXHC and SHETA genes of GDZC strains were 1007 and 971 bp,respectively. The nucleotide and amino acid sequences analysis showed that EXHC gene exhibited homology 100.0% with Staphylococcus hyicus Denmark strain (AF515455) and Staphylococcus sciuri Hebei strain (JF755400),while shared low homology,3.3% to 53.9% and 7.9% to 44.2% with exfoliative toxin of other Staphylococci stains based on nucleotide and amino acid sequence,respectively. There were 31 nucleotide mutations in the conserved region of SHETA gene,the nucleotide and amino acid sequence homologies with the Japan isolates (AB036768) were 96.2% and 98.4%,respectively. Using the DNAStar software to analyze the structure of proteins ecoded by EXHC and SHETA genes,the results showed that protein encoded by gene EXHC was hydrophilic protein,and protein encoded by gene SHETA was hydrophobic and poor antigenicity. This study amplified the EXHC and SHETA genes from Staphylococcus hyicus GDZC strains and predicted the protein structure successfully,and confirmed Staphylococcus hyicus from China could carry two toxins genes EXHC and SHETA,provided a theoretical basis for further study of the Staphylococcus hyicus pathogenic mechanism and interaction between toxins.

Key words: Staphylococcus hyicus; exfoliative toxins; EXHC; SHETA

CLC Number:

S858.28

ZHANG Le-yi, LI Yan, CAI Ru-jian, JIANG Zhi-yong, LIU Yan-ling, SONG Chang-xu. Cloning and Sequence Analysis of EXHC and SHETA Genes of Staphylococcus hyicus and Prediction of Protein Structure[J]. , 2014, 41(10): 17-22.

References

1 刘忠行,宋金成,王腾,等. 仔猪渗出性皮炎的病原分离鉴定与防控[J]. 中国兽医杂志,2013,49(7):43~45.
2 杨彩娟,蒋志勇,陈德坤,等. 猪葡萄球菌感染裸鼠及BALB/c小鼠模型的研究[J]. 中国动物检疫,2008,25(5):26~28.
3 苏小齐,王永强,李海花,等. 猪源松鼠葡萄球菌脱皮毒素抗体检测方法的建立与应用[J]. 中国兽医杂志,2013,49(12):7~13.
4 陈亚强,张乐宜,宋长绪,等. 猪葡萄球菌脱落毒素多重PCR检测方法的建立与初步应用[J]. 中国兽医学报,2012,32(10):1452~1456.
5 罗来鑫,童战江,张家峥,等. 猪渗出性皮炎及其防治[J]. 中国畜牧兽医,2007,34(11):96~98.
6 唐娜,莫玲,孙翠平,等. 一株鸡源猪葡萄球菌的分离与鉴定[J]. 中国预防兽医学报,2012,34(11):890~893.
7 睢艳平,李伟,任艳丽. 仔猪渗出性皮炎的实验室诊断[J]. 中国畜牧兽医,2006,33(10):13~16.
8 Ahrens P,Andresen L O. Cloning and sequence analysis of genes encoding Staphylococcus hyicus exfoliative toxin types A,B,C,and D[J]. Journal of Bacteriology,2004,186(6):1833~1837.
9 Andresen L O. Production of exfoliative toxin by isolates of Staphylococcus hyicus from different countries[J]. The Veterinary Record,2005,157(10):376~378.
10 Futagawa-Saito K,Ba-Thein W,Higuchi T,et al. Nationwide molecular surveillance of exfoliative toxigenic Staphylococcus hyicus on pig farms across Japan[J]. Veterinary Microbiology,2007,124(33):370~374.
11 Kanbar T,Voytenko A V,Gintsburg A L,et al. Prevalence of genes encoding exfoliative toxins among Staphylococcus hyicus isolated in Russia and Germany[J]. J Vet Med B Infect Dis Vet Public Health,2006,53(12):429~433.
12 Li H,Wang Y,Ding L,et al. Staphylococcus sciuri exfoliative toxin C (ExhC)is a necrosis inducer for mammalian cells[J]. PLoS One,2011,6(7):e23145.
13 Park J,Friendship R M,Poljak Z,et al. An investigation of exudative epidermitis (greasy pig disease) and antimicrobial resistance patterns of Staphylococcus hyicus and Staphylococcus aureus isolated from clinical cases[J]. Can Vet J,2013,54(2):139~144.
14 Sato H,Watanabe T,Murata Y,et al. New exfoliative toxin produced by a plasmid-carrying strain of Staphylococcus hyicus[J]. Infection and Immunity,1999,67(8):4014~4018.

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Cloning and Sequence Analysis of EXHC and SHETA Genes of Staphylococcus hyicus and Prediction of Protein Structure

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