QuEChERS-UPLC-MS/MS快速测定生鲜牛乳中的氟喹诺酮类药物残留

Abstract

Abstract: An UPLC-MS/MS method was developed and established for rapid determination of fluoroquinolones residues in raw bovine milk. The sample was extracted by QuEChERS extraction Kit, and cleaned up by QuEChERS clean-up tube. An aliquot sample of 5 mL was concentrated by nitrogen gas. The analytes were determined by UPLC-MS/MS and quantified by stable isotope internal standard. The results showed good linearity in the ranges of 2.0 to 100 μg/kg for all fluoroquinolones. The method recoveries were between 80% and 120%, and also the precisions were lower than 20%. The method is simple, rapid, and sensitive. It is suitable for screening of fluoroquinolones residues in raw bovine milk and quantitative determination.

Key words: UPLC-MS/MS; QuEChERS; fluoroquinolone; bovine milk; residue

CLC Number:

S859.84

QU Bin, ZHU Zhi-qian, LU Gui-ping, JIANG Tian-mei, GENG Shi-wei, GUO Liang-xue. Rapid Determination of Fluoroquinolones Residues in Raw Bovine Milk by UPLC-MS/MS via QuEChERS Pretreatment Method[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(S1): 50-54.

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Rapid Determination of Fluoroquinolones Residues in Raw Bovine Milk by UPLC-MS/MS via QuEChERS Pretreatment Method

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