Abstract: The clone vector pEASY-Blunt-F with fusion gene of canine distemper virus(CDV) isolated from vaccine strain was successfully constructed by T-A clone technique and was digested with restriction enzyme of BamHⅠ. The F gene was cloned into eukaryotic expression vector pSCA1. Finally a recombinant plasmid named pSCA1-F was constructed and identified by PCR, restriction enzyme analysis and sequencing. pSCA1-F was transfected into BHK-21 cells by using X-tremeGENE 9 DNA Transfection Reagent,IFA and Western blotting were performed to detect F expressions,the results showed that F gene had expressed in the transfected cells.Furthermore,apoptosis assay confirmed that pSCA1-F could induce the transfected cells apoptosis.

Key words: DNA vaccine; canine distemper virus; F gene; SFV-replicon; apoptosis