藏绵羊乳腺乳铁蛋白的克隆及序列分析

Abstract

Abstract: RT-PCR method was used to clone the full length of lactoferrin (LF) cDNA sequence in Tibetan sheep at Ruoergai county of Sichuan province. Nucleotide sequence and structure of LF amino acid sequence were deduced by bioinformatics software. The results showed that LF gene was 2127 bp in length and encoded 708 amino acids, including a signal peptide of 19 amino acids at its N terminal. Isoelectric point (pI) of this protein was 8.4, and the molecule mass of the predicted protein was 77.2 ku. Semi-quantitative RT-PCR results indicated that LF gene was expressed in mammary, trachea, lymph, liver, lachrymal and spleen, but not in lung. In conclusion,LF gene in Tibetan sheep had been successfully cloned and the nucleotide and amino acid sequence of LF and Lfc were analysed in this study, it laid a foundation for further research.

Key words: Tibetan sheep; LF gene; Lfc; clone; sequence analysis

CLC Number:

Q785

LI Jian-bo, JIANG Ming-feng, WANG Yong. Cloning and Sequence Analysis of Lactoferrin Gene in Tibetan Sheep[J]. , 2013, 40(9): 86-90.

References

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Cloning and Sequence Analysis of Lactoferrin Gene in Tibetan Sheep

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