金荞麦超微粉对小鼠免疫功能的影响

Abstract

Abstract: To study the effect of Fagopyrum dibotrys superfines powder on immune function and weight of mice,60 Kunming mice were divided into 4 groups,high, middle and low dose groups of mice were gavaged with the drug at a dose of 1.75, 1.25 and 0.75 g/(kg·BW) once a day for 7 consecutive days, repectively, the mice of control group were administered with the same volume of saline. 12 h after the last once, mice were injected into the muscle with Newcastle disease vaccine,then we got the peripheral blood on day 7, 14 and 21 after the medicine administrate. The enhancement of innate immune responses was evaluated by using of CCK-8 method, hemagglutination and hemagglutination inhibition test, macrophage phagocytic function test and organ coefficient method, and record the data of weight. In middle dose Fagopyrum dibotrys superfines powder group, the level of lymphocytes proliferative capacity, serum antibody, peritoneal macrophage phagocytosis and the index of immune organs were extremly significantly enhanced(P<0.01), and the weight was also extremly significantly increased(P<0.01). Fagopyrum dibotrys superfines powder could significantly regulate the immunity function and improve the weight in mice.

Key words: Fagopyrum dibotrys; superfines powder; mouse; immune function; growth

CLC Number:

S852.4

WANG Hang, TANG Cheng, YUE Hua, WANG Hong-ge, WANG Cheng-long. Study on the Effect of Fagopyrum dibotorys Superfines Powder on Immune Function of Mice[J]. , 2013, 40(9): 165-168.

References

1 王延树,张芳.血凝和血凝抑制试验操作要领[J].中国动物保健,2009(8):75~76.
2 邓蓉,梁应林,张定红,等.饲用金荞麦对猪屠宰性能和肉质的影响[J]. 黑龙江畜牧兽医,2012,2(1):68~70.
3 付绍玲.小鼠巨噬细胞吞噬功能测定实验方法探讨[J]. 湖北民族学院学报, 2008,25(4):48.
4 冯黎莎.金荞麦的抑菌活性研究[J].武汉植物学研究,2006,24(30):240~244.
5 汪春彦,吴常青,江勤,等.金荞麦对克雷伯杆菌肺炎大鼠心肌损伤中炎症细胞因子表达的影响[J].安徽医科大学学报,2011,46(1) : 44~48.
6 何昭阳,胡桂学,王春风.动物免疫学试验技术[M].长春:吉林科学技术出版社,2002.
7 陈冬金,黄志坚,陈强.复方黄芩颗粒剂对小鼠免疫功能的影响[J].江西农业大学学报,2007,29(5):809~812.
8 陈忠伟, 高建峰, 胡庭俊,等.马尾藻多糖对小鼠免疫功能调节作用的研究[J]. 中国畜牧兽医,2009,36(1):57~59.
9 陈晓峰,顾振纶.金养麦抗肿瘤作用研究进展[J].中草药,2000,31(9):715~718.
10 陈晓锋,顾振纶,杨海华,等.金荞麦Fr4对小鼠Lewis肺癌细胞MMP-9、TIMP-1蛋白表达的影响[J].苏州大学学报(医学版),2005,25(3):383.
11 周玉岩,乔红杰.金荞麦提取物体外抗菌活性研究[J].中兽医医药杂志,2009,5:44~46.
12 国家药典委员会.中国药典[S].北京:中国医药科技出版社,2010.
13 林忠宁,董胜璋,董书芸,等. MTT法检测T淋巴细胞增殖功能的方法学探讨与应用[J].中国卫生检验杂志,2000,10(1):8~10.
14 侯春梅,李新颖,叶伟亮,等.MTT法和CCK-8法检测悬浮细胞增殖的比较[J].军事医学科学院院刊,2009,33(4):400~401.
15 段波,赵兴中,李星国,等.超微粉制备技术的现状与展望[J]. 材料工程,1994,6(2):24~27.
16 程友斌,潘洪林.金荞麦抗炎活性部位筛选研究[J].时珍国医国药,2009,20(9):2219~2220.
17 盛华刚,朱立俏,林桂涛.金荞麦的化学成分与药理作用研究进展[J]. 西北药学杂志,2011,4(2):26~29.
18 Li J F,Li C.The influence of antiflammin-1 on adhesion between neutrophils and endothelial cells induced by LPS[J] Hunan Province Physical Science Papers in the Assembly,2008,11(1):34~37.
19 Wang K J,Zhang Y J,Yang C R,et al.Antioxidant phenolic constituents from Fagopyrum dibotrys[J].J Ethnopharmocol,2005,99(2):259~264.

[1]	LI Yanghui, XU Tao, ZHANG Shuxian, LIU Ke, JIA Tianqing, CHEN Ning, YIN Junliang, XI Linqiao, WANG Dong, ZHOU Xiaoling. Effect of Replacing Wheat Straw with Cumin Straw on Growth Performance, Slaughter Performance and Plasma Biochemical Parameters of Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2296-2304.
[2]	WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.
[3]	LI Jie, CHEN Chuwen, ZHAO Ruipeng, LIU Yuan, LI Zhixiong. Research Progress on Long Non-coding RNA of Muscle Development in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2427-2438.
[4]	OUYANG Kun, WEI Limin, SUN Ruiping, XIE Yali, QI Qi, LI Xiang, LIU Quanwei, CHAO Zhe, ZHANG Yongliang, CHEN Ting. Effects of Curcumin on Growth Performance,Feed Apparent Digestibility and Serum Biochemical Indexes in Hainan Tunchang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1867-1875.
[5]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[6]	HU Honglian, LI Chao, CHEN Liqing, SONG Liwen, ZHAO Meng, ZHANG Jianxia, WANG Dian, GAO Min. Effects of Dietary β-carotene Supplementation on Lactation Performance, Blood Routine,Serum Biochemical and Immune Indexes of Dairy Cows during the Lactating Peak Period [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1375-1384.
[7]	GUO Yaru, LI Kexin, WANG Li, LIU Jinyu, SHI Lei, REN Youshe. Effects of Selenium on Lead-Induced Apoptosis of Mouse Leydig Cells in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1404-1411.
[8]	REN Jiangang, GUAN Peng, HUANG Jianzhi, SHENG Yueyun, BAO Tiezhu, MA Zhiping, QIAN Wenxi. Effects of Dietary Energy Level on Growth-fattening Performance and Serum Biochemical Indices of Angus Bulls [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 966-976.
[9]	XIONG Taidi, JIANG Shouqun, ZHANG Huihua, ZHU Cui. Research Progress on Transportation Stress and Its Mitigation Measures in Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 977-988.
[10]	LIU Zhen, ZHOU Xueqin, XU Fei, LI Zhefeng, SUN Hanxue, LI Guoqin, TAO Zhengrong, GUAN Mei, ZHENG Qiang, DIAO Xinping, LU Lizhi. Effects of Coated Sodium Butyrate on Growing Performance,Slaughter Performance, Immune Organ Index and Digestive Enzyme Activity of Breeding Pigeons and Squabs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 998-1006.
[11]	LI Bin, SHI Zhengling, ZENG Ziyou, HUANG Yingyan, LI Lin, DU Zilai, JI Yifan, WANG Di, YU Jie, MEI Shaofeng. Effects of Chicory on Growth Performance,Slaughter Traits and Meat Quality of Mahuang Broilers with Different Gender [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 500-511.
[12]	LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.
[13]	ZHAO Luyuan, ZOU Chenhao, CAO Jiu'ai, GUO Yanshan, LIU Lijun, ZHAN Xiu'an, LIN Gang, LIU Bing, YU Dongyou. Effects of Low Level of Compound Organic Trace Minerals on Serum Biochemistry,Immune Function and Antioxidant Capacity of Meat Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 86-96.
[14]	WANG Yuan, WANG Wenwen, AN Xiaoping, QI Jingwei. Effects of Dietary Fermented Soybean Meal Replacement on Growth Performance, Digestive Enzyme,and Microbita Composition in Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 115-124.
[15]	LIN Xiajing, ZHENG Chuntian, XI Pengbin, CHENG Zhonggang, DING Fayuan, RUAN Dong, WANG Yibing, JIANG Shouqun. Dietary Threonine Requirement of Yellow-feathered Broilers Aged From 22 to 42 Days [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 134-142.

Study on the Effect of Fagopyrum dibotorys Superfines Powder on Immune Function of Mice

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments