不同浓度秋水仙素和nocodazole对绒山羊成纤维细胞周期同步化的影响

Abstract

Abstract: This experiment was conducted to explore optimized conditions to make more Cashmere goat fibroblasts which had been treated in G2/M. In the experiment, the colchicine and nocodazole reagents were used in the processing of Cashmere goat fibroblast cell cycle synchronization, and the number of the cell in G2/M was measured by flow cytometry to observe the optimal concentration and action time. The results showed that the colchicine concentration should not be too large;furthermore, the time should not be too long in treatment of colchicines. In addition, under the same conditions, the treatment of nocodazole worked better than colchicine in synchronization. The optimum processing conditions included the concentration was 300 ng/mL and the action time was 24 h. Then the percentage of fibroblast in G2/M accounted for 15.10%. The results suggested that the effect of nocodazole reagents was more pronounced for Cashmere goats fibroblasts, when conducting the cell cycle synchronization processing.

Key words: Cashmere goat; fibroblast; colchicine; nocodazole; cell cycle synchronization; flow cytometry

CLC Number:

Q813

YANG Hui-ru, XIAO Hong-mei, CAI Ting, JU Hong-li, YU Xin-lei, LIU Zhi-hong, LI Jin-quan, WANG Zhi-xin. The Effect of Colchicine and Nocodazole in Different Concentrations on Cycle Synchronization of Fibroblast in Cashmere Goat[J]. , 2013, 40(9): 136-139.

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The Effect of Colchicine and Nocodazole in Different Concentrations on Cycle Synchronization of Fibroblast in Cashmere Goat

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