Abstract: The study on the evolution and expression pattern of cardiac α-actin in Luxi Huang cattle might provide some information to understand the function of cardiac α-actin. We did the bioinformatics analysis to show the evolution information about the cardiac α-actin, and we did the Western blotting and immunofluorescence experiments to show the expression pattern of cardiac α-actin. The results showed that the cardiac α-actin was expressed strongly in adult heart tissues, and at the same time it was expressed weakly in skeletal muscle. The expressed pattern suggested that the cardiac α-actin function was not only in heart development but in skeletal muscle development.

Key words: cardiac α-actin; expression pattern; skeletal muscle; heart