胚胎干细胞向生殖细胞分化的研究进展

Abstract

Abstract: The germ cells come from the primordial germ cells(PGCs), in vivo differentiation mechanism of which has been basically understood. With the development of germ cell studies, in vitro induction of germ cells has become the reality. Embryonic stem cells(ESCs) can be induced to differentiate into epiblast-like cells, then into primordial germ-like or primordial germ cells. When transferred into fetal gland they have the abilities to undergo meiosis and create sperm; when transferred together with ovum into gonad of newly born mouse lacking of seminiferous tubule, they can engender healthy offspring. Therefore, induction of germ cells in vitro further elucidates the mechanism of the ESCs differentiating into germ cells, and lays the foundation for more effective utilization of stem cells to benefit mankind.

Key words: embryonic stem cells; germ cells; differentiation

CLC Number:

Q813

RAO Jia-hui, ZHOU Xu. Research Progress of Embryonic Stem Cells Differentiating into Germ Cell[J]. , 2013, 40(3): 137-140.

References

1 朱士恩.家畜繁殖学[M].第5版.北京:中国农业出版社,2011.
2 李松,高佩安,江新泉,等.从原始生殖细胞中分离克隆胚胎干细胞的研究[J].中国畜牧兽医,2008,35(6):64～65.
3 张红卫.发育生物学[M].第2版.北京:高等教育出版社,2006.
4 董云玲,刘锦云,王克华.胚胎干细胞向生殖细胞分化的研究进展[J].中国计划生育学杂志,2011,19(4):247～249.
5 Chia N Y,Chan Y S,Feng B,et al.A genone-wide RNAi screen reveals determinants of human embryonic stem cell identity.Nature,2010,468(7321):316～320.
6 Clark A T, Bodnar M S, Fox M, et al. Spontaneous differentiation of germ cells from human embryonic stem cells in vitro. Hum Mol Genet,2004,13(7):727～739.
7 Danner S,Kajahn J,Geismann C,et al. Derivation of oocyte-like cells from a clonal pancreatic stem cell line. Mol Hum Reprod,2007,13(1):11～20.
8 Dyce P W, Wen L, Li J. In vitro germline potential of stem cells derived from fetal porcine skin. Nat Cell Biol,2006,8(4):384～390.
9 Geijsen N, Horoschak M, Kim K, et al. Derivation of embryonic germ cells and male gametes from embryonic stem cells. Nature, 2004,427(6970):148～154.
10 Greber B,Wu G,Bememann C,et al.Conserved and divergent roles of FGF signaling in mouse epiblast stem cells and human embryonic stem cells.Cell Stem Cell,2010,6(3):215～226.
11 Hübner K,Fuhrmann G,Christenson L K,et al. Derivation of oocytes from mouse embryonic stem cells. Science,2003,300(5623): 1251～1256.
12 Hayashi K,Ohta H,Kurimoto K,et al.Reconstituion of the mouse germ cell spericification pathway in culture by pluripotent stem cells.Cell,2011,146(4):519～532.
13 Kerkis A,Fonseca S A,Serafim R C,et al. In vitro differentiation of male mouse embryonic stem cells into both presumptive sperm cells and oocytes.Cloning Stem Cell,2007,9(4):535～548.
14 Kim Y,Turner D,Nelson J,et al. Production of donor-derived sperm after spermatogonial stem cell transplantation in the dog. Reproduction,2008,136(6):823～831.
15 Kurimoto K,Yabuta Y,Ohinata Y,et al. Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ lineage in mice.Genes Dev,2008,22(12):1617～1635.
16 Labosky P A,Hogan B L. Mouse primordialgerm cells:Isolation and in vitro culture. Methods Mol Biol,2008,461:187～199.
17 Lue Y,Erkkila K,Liu P Y,et al. Fate of bone marrow stem cells transplanted into the testis:Potential implication for men with testicular failure. Am J Pathol,2007,170(3):899～908.
18 Nakagawa M,Takizawa N,Narita M,et al.Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci USA,2010,107(32),14152～14157.
19 Nayernia K,Nolte J,Michelmann H W,et al. In vitro differentiated embryonic stem cell give rise to male gametes that can generate offspring mice. Dev Cell,2006a,11(1):125～132.
20 Nayernia K, Lee J H, Drusenheimer N, et al. Derivation of male germ cells from bone marrow stem cells. Lab Invest,2006b,86(7): 654～663.
21 Nichols J,Silva J,Roode M,et al.Suppression of Erk signaling promotes ground state pluripotency in the mouse embryo.Development,2009,136(19):3215～3222.
22 Ohinata Y,Ohta H,Shigeta M,et al.A signaling principle for the germ cell lineage in mice.Cell,2009,137:571～584.
23 Rathjen J,Lake J A,Bettess M D,et al. Formation of primitive ectoderm like cell population,EPL cells,from ES cells in response to biologically derived factors. J Cell Sci,1999,112:601～612.
24 Saitou M,Yamaji M. Germ cell specification in mice:Signaling,transcription regulation,and epigenetic consequences. Reproduction,2010,139:931～942.
25 Thomas P Z,James A T.A germ cell origin of embryonic stem cells.Development,2005,132:227～233.
26 Thomson M,Liu J S Y,Zou L N,et al.Pluripotency factors in embryonic stem cells regulate differentiation into germ layers.cell,2011,145:875～889.
27 Toyooka Y,Tsunekawa N,Akasu R,et al. Embryonic stem cells can form germ cells in vitro. Proc Natl Acad Sci USA, 2003,100(20):11457～11462.
28 Yamano N,Kimura T,Watanabe-Kushima S,et al.Metastable primordial germ cell-like state induced from mouse embryonic stem cells by AKT activation.Biochem Biophys Res Commun,2010,392(3):311～316.
29 Yu Z,Ji P,Cao J,et al. Dazl promotes germ cell differentiation from embryonic stem cells. J Mol Cell Biol,2009,1(2):93～103.
30 Zhou Q,Li Y,Nie R,et al. Expression of stimulated by retinoic acid gene 8 (Stra8) and maturation ofmurine gonocytes and spematogonia induced by retinoic acid in vitro.BiolReprod,2008,78(3):537～545.
31 Zou K,Yuan Z,Yang Z,et al. Production of offspring from a germline stem cell line derived from neonatal ovaries. Nat Cell Biol,2009,11(5):631～636.

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Research Progress of Embryonic Stem Cells Differentiating into Germ Cell

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