山羊前列腺素内过氧化物合酶2基因的多态性检测及生物信息学分析

Abstract

Abstract: In this study, Guizhou White goat,Guizhou Black goat and Qianbei Ma goat from Guizhou native goat breeds, and Guanzhong Dairy goat and inner Mongolia cashmere goat from other province were selected for testing subjects, the technology of direct sequencing of PCR products was used to detect the single nucleotide polymorphism of prostaglandin-endoperoxide synthase 2(PTGS2) gene in goats. The consequence showed that six SNPs(A96G,C20T,A239G,T192C,T164A and G91C) were found, among these SNPs, A96G and T164A lay in the exon 7 and exon 8, which were sense mutation and the others lay in intron. Bioinformatics analysis revealed that the two SNPs in the exon 7 and exon 8 lead to the alteration of secondary structure of mRNA.

Key words: goat; PTGS2 gene; SNP; bioinformatics

CLC Number:

S813.3

SONG Tao-wei, LUO Wei-xing, CAI Hui-fen, LIU Ruo-yu, CHEN Zhi, HUI Yan-ting. Polymorphism Detection and Bioinformatics Analysis of Prostaglandin-endoperoxide Synthase 2 Gene in Goat[J]. , 2013, 40(2): 121-125.

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Polymorphism Detection and Bioinformatics Analysis of Prostaglandin-endoperoxide Synthase 2 Gene in Goat

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