牛奶中β-内酰胺类和四环素类药物胶体金快速检测方法的建立

Abstract

Abstract: Colloidal gold immunochromatographic was uesd for the determination of β-lactams and tetracyclines in milk. Tetracyclines hapten was obtained by chemical synthesis. Immunogen and envelope antigen were prepared by cephalosporins and tetracyclines hapten coupled to carrier protein. Monoclonal antibodies were obtained by immunogen immunizing mice. A rapid and simple colloidal gold immunochromatographic assay was established for the determination of β-lactams and tetracyclines in milk. 12 kinds of β-lactams and 4 kinds of tetracyclines were detected at the same time by the strip. The milk sample could be used to be detected without dilution. The detection time could be 3 to 5 min. The method could be efficiently used for mass samples screening in primary laboratory.

Key words: β-lactams; tetracyclines; colloidal gold immunochromatographic assay; rapid detection strip; milk

CLC Number:

TS201.6

NIE Wen-ying, TANG Cheng-ming, WANG Jian-xia, LUO Xiao-qin, FENG Cai-wei. Development of Colloidal Gold Rapid Detection Method of β-lactams and Tetracyclines in Milk[J]. , 2013, 40(12): 70-75.

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Development of Colloidal Gold Rapid Detection Method of β-lactams and Tetracyclines in Milk

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