›› 2013, Vol. 40 ›› Issue (12): 48-51.

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Sequence Analysis of VP2 Gene of Four Infectious Bursal Disease Virus Strains

WANG Shou-shan, MAO Ya-yuan, ZHANG Li-xia, KANG Ya-nan, SUN Mei-yu, CHEN Bing, LV Mao-jie, HE Zhao-qing, YANG Bao-shou   

  1. Ringpu Biological Research Institute, Tianjin Ringpu Bio-technology Co., Ltd., Tianjin 300308, China
  • Received:2013-05-15 Online:2013-12-20 Published:2014-02-11

Abstract: Four infectious bursal disease virus (IBDV) strains were isolated from Hebei (HB), Hubei (HUB), Shandong (SD) and Shanxi (SX), respectively. Gene analysis showed that the seven peptide areas of HUB, SD and SX were S-A-S-S-W-G-S (aa 326S to 332S), and 222(A), 256(I), 294(I) and 299(S) determined the three strains were vvIBDV. However, the seven peptide area of HB was S-W-S-A-R-G-S (aa 326S to 332S), 222(P), 256(V), 294(L) and 299(N), which showed HB was the weak strain. Evolutionary analysis indicated that HUB, SD and SX virus strains were located on the branch of vvIBDV and were similar with the most IBDV strains, which were isolated in 2011, the homologies were all above 96%. However, HB had close genetic distance with attenuated vaccine strains D78 and B87. IBDV was popular in the mixed style currently in China, but mainly was vvIBDV.

Key words: infectious bursal disease virus; VP2 gene; sequence analysis

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