Abstract: According to OmpH gene of Pasteurella multocida published in GenBank, a pair of primers was designed. OmpH gene of 8 avian Pasteurella multocida strains and three capsular-type reference strains (A, B, D) were amplified by PCR and sequenced. The results showed that the ORF of OmpH gene were from 1002 to 1071 bp.The predicted maturation proteins were composed of 314 to 337 amino acids except 20-amino-acid signal peptide. The molecular weights of maturation proteins were from 33.76 to 37.04 ku. The alignment data indicated that the nucleotide sequence homologies were 84.9% to 100.0%, and the deduced amino acid homologies were 81.5% to 100.0% compared with 15 reference OmpH sequence of Pasteurella multocida published in GenBank.The homomologies of OmpH sequences of six avain Pasteurella multocida strains isolated from China were 100.0%. The results revealed that the OmpH genes of avain Pasteurella multocida strains isolated from China were very conservative.

Key words: avian Pasteurella multocida; OmpH gene; sequence analysis; homology