猪骨形态发生蛋白-6基因多态性及其与产仔性能的关联性分析

Abstract

Abstract: In this research, we studied the genetic variation of porcine bone morphogenetic protein 6(BMP-6) gene and the associations between the genetic variation and the litter size. Genetic variation of BMP-6 gene was detected by PCR-SSCP in 268 individuals from five swine breeds (Wild boar, Min pig, Landrace, Beijing Black pig and French Yorkshire), and association analysis were carried out to evaluate the effects of genotypes on litter size in French Yorkshire. The G1104A transition mutation, a synonymous mutation, was found within CDS of BMP-6 gene, and there were three genotypes of AA, AB and BB in all breeds. The A allele frequency was greater in Wild boar and Min pig, whereas the B allele was superior in Landrace, Beijing Black pig and French Yorkshire. The results showed that there were significant differences among sows with different BMP-6 genotypes in TNB and NBA, which implied that the G1104A mutation within BMP-6 gene had the significant influence on litter size in swine.

Key words: pig; BMP-6 gene; polymorphism; litter size

CLC Number:

S814.8

CUI Yu, YANG Shao-cheng, LIU Di, HUA Yu-ping. The Polymorphism of Porcine BMP-6 Gene and its Association with the Litter Sizes[J]. , 2013, 40(12): 137-141.

References

1 郭良勇, 闫学军, 王爱国, 等. 猪ITGB1基因多态性与产仔数的关联性分析[J]. 中国畜牧杂志, 2012, 48(21): 22~25.
2 吴双庆. 骨形态发生蛋白-6的研究进展[J]. 国外医学遗传学分册, 2003, 26(6): 350~353.
3 储明星,肖杰文,李学伟,等. 4个山羊品种BMP6基因部分片段的多态性分析[J]. 中国畜牧兽医, 2008, 35(1): 55~58.
4 Arndt S, Maegdefrau U, Dorn C, et al. Iron-induced expression of bone morphogenic protein 6 in intestinal cells is the main regulator of hepatic hepcidin expression in vivo[J]. Gastroenterology, 2010, 138(1): 372~382.
5 Ara J, See J, Mamontov P, et al. Bone morphogenetic proteins 4, 6, and 7 are up-regulated in mouse spinal cord during experimental autoimmune encephalomyelitis[J]. J Neurosci Res, 2008, 86(1): 125~135.
6 Brankin V, Quinn R L, Webb R, et al. BMP-2 and -6 modulate porcine theca cell function alone and co-cultured with granulose cells[J]. Domestic Animal Endocrinology, 2005, 29(4): 593~604.
7 Campbell B K, Kendall N R, Baird D T. Effect of direct ovarian infusion of bone morphogenetic protein 6 (BMP-6) on ovarian function in sheep[J]. Biol Reprod, 2009, 81(5): 1016~1023.
8 Darby S, Cross S S, Brown N J, et al. BMP-6 over-expression in prostate cancer is associated with increased Id-1 protein and a more invasive phenotype[J]. J Pathol, 2008, 214(3): 394~404.
9 Diekman B O, Estes B T, Guilak F. The effects of BMP-6 overexpression on adipose stem cell chondrogenesis: Interactions with dexamethasone and exogenous growth factors[J]. Journal of Biomedical Materials Research Part A, 2010, 93(3): 994~1003.
10 Erickson G F, Fuqua L, Shimasaki S. Analysis of spatial and temporal expression patterns of bone morphogenetic protein family members in the rat uterus over the estrous cycle[J]. J Endocrinol, 2004, 182(2): 203~217.
11 Glister C, Kemp C F, Knight P G. Bone morphogenetic protein (BMP) ligands and receptors in bovine ovarian follicle cells: Actions of BMP-4, -6 and -7 on granulosa cells and differential modulation of Smad-1 phosphorylation by follistatin[J]. Reproduction, 2004, 127(2): 239~254.
12 Hogg K, Etherington S L, Young J M, et al. Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family[J]. Endocrinology, 2010, 151(3): 1247~1256.
13 Juengel J L, McNatty K P. The role of proteins of the transforming growth factor-β superfamily in the intraovarian regulation of follicular development[J]. Hum Reprod Update, 2005, 11(2): 144~161.
14 Kayani A R, Glister C, Knight P G. Evidence for an inhibitory role of bone morphogenetic protein(s) in the follicular-luteal transition in cattle[J]. Reproduction,2009, 137(1): 67~78.
15 Meynard D, Kautz L, Darnaud V, et al. Lack of the bone morphogenetic protein BMP6 induces massive iron overload[J]. Nat Genet, 2009, 41(4): 478~481.
16 Otsuka F, Moore R K, Shimasaki S. Biological function and cellular mechanism of bone morphogenetic protein-6 in the ovary[J]. J Biol Chem, 2001, 276(35): 32889~32895.
17 Paradis F, Novak S, Murdoch G K, et al. Temporal regulation of BMP2, BMP6, BMP15, GDF9, BMPR1A, BMPR1B, BMPR2 and TGFBR1 mRNA expression in the oocyte, granulosa and theca cells of developing preovulatory follicles in the pig[J]. Reproduction,2009, 138(1): 115~129.
18 Park S S, Park M J, Joo B S, et al. Improvement of ovarian response and oocyte quality of aged female by administration of bone morphogenetic protein-6 in a mouse model[J]. Reprod Biol Endocrinol, 2012, 10: 117.
19 Shi J, Yoshino O, Osuga Y, et al. Bone morphogenetic protein-6 stimulates gene expression of follicle-stimulating hormone receptor, inhibin/activin beta subunits, and anti-müllerian hormone in human granulosa cells[J]. Fertil Steril, 2009, 92(5): 1794~1798.
20 Sugiura K, Su Y Q, Eppig J J. Does bone morphogenetic protein 6 (BMP-6) affect female fertility in the mouse[J]. Biol Reprod, 2010, 83(6): 997~1004.

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The Polymorphism of Porcine BMP-6 Gene and its Association with the Litter Sizes

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