新城疫病毒和鸭瘟病毒二重PCR检测方法的建立及初步应用

Abstract

Abstract: Two sets of specific primers were designed according to the sequences of Newcastle diseases virus (NDV) and duck plague virus (DPV) in GenBank. It was shown that all samples containg NDV and DPV could be amplified into two specific bands, 419 bp for NDV and 602 bp for DPV by this duplex PCR, but no specific band was amplified from other avian pathogenic virus and bacteria. As little as 0.4 pg of NDV RNA and 126 pg of DPV DNA could be detected by this duplex PCR.

Key words: Newcastle diseases virus; duck plague virus; duplex PCR

CLC Number:

S858.32

CHEN An-li, XIE Zhi-xun, XIE Li-ji, XIE Zhi-qin, PANG Yao-shan, DENG Xian-wen, LIU Jia-bo, PENG Yi, FAN Qin, LUO Si-si. Development and Application of a Duplex PCR Assay for Detection of Newcastle Diseases Virus and Duck Plague Virus[J]. , 2013, 40(11): 192-195.

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Development and Application of a Duplex PCR Assay for Detection of Newcastle Diseases Virus and Duck Plague Virus

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