绵羊肺炎支原体和精氨酸支原体双重PCR检测方法的建立及应用

doi:10.16431/j.cnki.1671-7236.2015.09.004

Abstract

Abstract: In order to establish a duplex PCR method for simultaneous detection of Mycoplasma ovipneumiae and Mycoplasma arginini, specific primers of Mycoplasma ovipneumiae and Mycoplasma arginini were designed, and evaluated its sensitivity and specificity after optimizing the reaction conditions of PCR.Then, a total of 40 nasal swabs were tested by duplex PCR.The assay could specifically amplify PCR fragments of 545 and 806 bp from Mycoplasma ovipneumiae and Mycoplasma arginini, respectively.While no PCR products were detected for other pathogens.The detection limits of the assay were determined to be 100 pg/μL for Mycoplasma ovipneumiae and 10 pg/μL for Mycoplasma arginini.The duplex PCR could detect Mycoplasma ovipneumiae and Mycoplasma arginini, and the coincidence rate could reach as high as 92.5% with enrichment culture about the 40 nasal swabs.The results suggested that the duplex PCR could be useful for clinical detection of Mycoplasma ovipneumiae and Mycoplasma arginini.

Key words: Mycoplasma ovipneumiae; Mycoplasma arginini; duplex PCR

CLC Number:

S858.26

ZHENG Jia-qi, HUANG Hai-bi, WANG Xiao-hui, LI Zhen-ya, XING Meng-en, HAO Yong-qing. Development and Application of a Duplex PCR Assay for Detecting Mycoplasma ovipneumiae and Mycoplasma arginini[J]. , 2015, 42(9): 2240-2245.

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Development and Application of a Duplex PCR Assay for Detecting Mycoplasma ovipneumiae and Mycoplasma arginini

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