安徽省五华鸡J亚群禽白血病病毒gp85基因分子序列特征分析

›› 2013, Vol. 40 ›› Issue (11): 195-200.

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Sequence Feature Analysis of gp85 Gene of Avian Leukosis Virus Subgroup J in Wuhua Chickens in Anhui Province

DAI Yin¹, ZHAO Rui-hong¹, HU Xiao-miao¹, ZHANG Dan-jun¹, XIA Song-lin², SHENG Chang-shu³, YANG Wen-chao³, ZHONG Guo-fa⁴

1. Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei 230031, China;
2. Wuhu Center for Animal Disease Control and Prevention, Wuhu 241000, China;
3. Center for Animal Disease Control and Prevention of Fanchang County, Fanchang 241200, China;
4. Wuhua Chicken Breed Protection Company of Fanchang County, Fanchang 241209, China

Received:2013-04-26 Online:2013-11-20 Published:2013-12-19

Abstract

Abstract: In order to further understand the structure,function and genetic variation of ALV-J gp85 gene,the gene was cloned by PCR in Anhui province,which named as ALV-J-WH. We compared ALV-J-WH with fifteen amino acid sequence of ALV-J gp85 genes in GenBank database. The results showed that the homology between ALV-J-WH and the other sequences ranged from 85.9% to 96.2%, the highest homology with ALV-J-WH were JN378888 sequences isolated from China. Evolution analysis further revealed that they had a close genetic relationship. Moreover,the deletion of a 10 amino acid was observed in the ALV-J-WH between position 223 and 224 of amino acid sequence. Alignment of the full-length sequences revealed 71 variable amino acid sites,10 sites had high mutation frequency,expecially sites in hr1 and hr2 domains. The results confirmed that ALV-J-WH and partial genes of strains isolated from China evolved from common strains,but the sequence was unique. ALV-J gp85 gene was highly variable,and some amino acids with high degree of mutation frequency might be action sites of ALV-J antibody under immune selective pressure.

Key words: avian leukosis virus subgroup J; gp85 gene; sequence feature

CLC Number:

S831

DAI Yin, ZHAO Rui-hong, HU Xiao-miao, ZHANG Dan-jun, XIA Song-lin, SHENG Chang-shu, YANG Wen-chao, ZHONG Guo-fa. Sequence Feature Analysis of gp85 Gene of Avian Leukosis Virus Subgroup J in Wuhua Chickens in Anhui Province[J]. , 2013, 40(11): 195-200.

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[2]	DAI Yin;ZHANG Dan-jun;HU Xiao-miao;ZHAO Rui-hong. DNA Cloning and Sequence Analysis of Partial env Gene of Avian Leukosis Virus Subgroup J in Wuhua Chicken Flocks [J]. , 2011, 38(12): 122-124.

Sequence Feature Analysis of gp85 Gene of Avian Leukosis Virus Subgroup J in Wuhua Chickens in Anhui Province

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