脂多糖染毒小鼠稳定内参基因的筛选

Abstract

Abstract: The aim of this study was to select ideal reference genes in mice incubated with lipopolysaccharides (LPS) stimulation. The mRNA expression of five reference genes of healthy mouse livers and 3, 6, 12, 18 h mouse livers incubated with LPS, named YWHAZ, HPRT1, PPIA, ACTB and 18S rRNA, were evaluated respectively, using fluorescence quantitative RT-PCR(qRT-PCR). The geNorm software analysis showed that under the condition of LPS stimulation, 18S rRNA did not show stable expression; the other four reference genes showed expression stability, among which PPIA and YWHAZ were the most stable. The result in this study provided a reference for studying the expression of genes by qRT-PCR under the interaction between LPS and mice.

Key words: reference genes; fluorescence quantitative RT-PCR(qRT-PCR); mice; lipopolysaccharides(LPS)

CLC Number:

ZHOU Hong-miao, YUE Hua, TANG Cheng, WU Qiao. Selection of Stable Reference Genes in Mice Incubated with LPS Stimulation[J]. , 2013, 40(10): 68-72.

References

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Selection of Stable Reference Genes in Mice Incubated with LPS Stimulation

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