鲤鱼肿瘤坏死因子受体相关因子6全长cDNA克隆及序列分析

Abstract

Abstract: The common carp tumor necrosis factor receptor-associated factor 6 (TRAF6) EST sequence was picked out from the cDNA library of peripheral blood leukocytes that was constructed. The cDNA library were separated from carp and stimulated with mitogen was screened by a probe labeled with DIG. The full-length TRAF6 cDNA was cloned from recombinant phages. Sequence analysis indicated that it encompasses with a 25 bp 5'-UTR and a 535 bp 3'-UTR,the open reading frame (ORF) of which was 1632 bp putatively coding 543 amino acids,and there were two motifs for mRNA instability ATTTA in the 3'-untranslated region. The predicted theoretical isoelectric point and molecular weight were 5.88 and 61.773 ku,respectively. Its nucleotide homology with carp TRAF6 from GenBank was up to 99%.The protein sequence analysis showed that it shared typical sequence features of the TRAF family.

Key words: common carp; TRAF6; clone; sequence analysis

CLC Number:

Q785

JIA Sheng-mei, SUN Zhen, FENG Xiang-ru, CHEN Yi-long, SHEN Xue-fei, ZHAI Xin-xin, ZHANG Jun-hui, YANG Zhen-guo, WANG Wen-dong, LU Qiang. Clonging and Sequence Analysis of Tumor Necrosis Factor Receptor-associated Factor 6 Full-length cDNA from Common Carp[J]. , 2013, 40(10): 42-46.

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Clonging and Sequence Analysis of Tumor Necrosis Factor Receptor-associated Factor 6 Full-length cDNA from Common Carp

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