动物源产CMY-2大肠杆菌分子流行病学研究

Abstract

Abstract: To investigate the epidemiology of CMY-2-producing E.coli from different animals throughout Guangdong province, 1293 isolates from pig, chicken, duck and goose from 2010 to 2011 were recruited in this study. All the isolates were screened for bla_CMY-2 gene by PCR and sequenced; the MICs of bla_CMY-2-positive isolates to 17 kinds of antimicrobial drugs were tested by agar dilution; conjugation experiments were performed on bla_CMY-2 harboring isolates to explore the transferability of bla_CMY-2gene to E.coli C600, and XbaⅠ-PFGE analysis were used to determine the transfer and spread manner of bla_CMY-2 among E.coli isolates. The results showed that 27(2.09%)of 1293 isolates carried bla_CMY-2,and were present multi-resistant phenotypes, in which the main one was AMP/CHL/TET/FLF/CTF/CTX/CAZ/CTR/GEN/CIP/ENR/NAL/OQX. The bla_CMY-2 gene in 14 of 27 isolates transferred successfully to E.coli C600 by plasmids, and most of them co-transferred with bla_TEM-1 and/or qnrS1. PFGE profiles showed that 17 different patterns were successfully got, and two from the same place were clonally related as well as another two isolates. CMY-2 encoding gene on a transferability plasmid or carried by other mobile element presumably was the major cause for the epidemiology of CMY-2-producing E.coli among food animals.

Key words: E.coli; resistance; animals; CMY-2

CLC Number:

S852.61

GUO Yu-fang, ZHAO Qiu-yun, JIANG Rui, REN Jin-cheng, YANG Ling, ZHEN Pan-pan, TANG Dian, JIANG Hong-xia. Epidemiology Investigation of CMY-2-producing E.coli from Animals[J]. , 2013, 40(10): 47-51.

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Epidemiology Investigation of CMY-2-producing E.coli from Animals

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