牦牛产志贺毒素大肠杆菌主要黏附因子相关基因的分子流行病学调查

Abstract

Abstract: The assay was aimed to investigate the prevalence of the major adhension factors in Shiga toxin-producing Escherichia coli from Yak.By using the method of PCR to detect the adhension factors of eae,saa,iha genes in the 70 STEC isolates from the healthy Yak in Sichuan Ganzi,Aba and other places,some of the adhension genes were cloned and sequenced. The results showed that the carried rates of saa and iha genes were 71.42%(50/70) and 78.57%(55/70) respectively in 70 STEC strains from Yak, none of the STEC isolates carried the eae gene(0/70), the homology of saa and iha genes was 100% and 93% to 99% respectively with the reference sequences in GenBank.STEC isolates from the healthy Yak didn’t have eae gene which encoded by LEE virulence island, the carried rates of other adhension virulence genes saa, iha was higher.

Key words: Yak; Shiga toxin-producing Escherichia coli; adhension gene

CLC Number:

S852.61

SONG Ding-zhou, YU Xue-hui, TANG Cheng, LI Jian, RAN Dan-dan. Molecular Epidemiological Investigation of Genes Related with the Major Adhesion Factors in Shiga Toxin-producing Escherichia coli from Yak[J]. , 2013, 40(1): 153-157.

References

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Molecular Epidemiological Investigation of Genes Related with the Major Adhesion Factors in Shiga Toxin-producing Escherichia coli from Yak

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