遗传背景和胎儿日龄对猪类胚胎生殖细胞培养的影响

Abstract

Abstract: Factors on culturing of embryonic gem cells (EG)in pigs were studied in order to establish porcine EG lines. In order to analysis genetic background influencing the culturing of the porcine embryonic germ cells, fetuses were collected from Wuzhishan pigs (WZSP) and White Minitype pigs respectively. Under the same culturing conditions, when the first time of EG colonies coming forth (T1),the first time of large quantities of EG colonies coming forth (T2),the first time of passage (T3),average passage intervals (T4),passages (P) were considered. The results showed that there was no notable difference in EG culturing between the two types of pigs(P>0.05). That was WZSP and White Minitype pigs were all suitable materials to establish porcine EG lines; In order to analysis fetal stages influencing the culturing of porcine EG, fetuses at 26 d, 27 d and 28 d were collected respectively, the length of the fetuses and the length of the fetal mesonephros were measured, the 5 indexes above mentioned were still considered, the results showed that under the same conditions, there was no notable difference in EG culturing using fetuses collected on 26 d, 27 d and 28 d respectively(P>0.05).

Key words: genetic background; fetal stage; pig; embryonic germ cells

CLC Number:

S828

DONG Xiao, FENG Shu-tang, WANG Hong-jun. The Effect on the Culturing of Porcine Embryonic Germ Cells by Genetic Background and Fetal Stage[J]. , 2013, (7): 137-142.

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The Effect on the Culturing of Porcine Embryonic Germ Cells by Genetic Background and Fetal Stage

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