5株猪圆环病毒2型全基因克隆和序列分析

Abstract

Abstract: To understand the prevalent trend and genetic variations of porcine circovirus type 2(PCV2) from pig farms in Guangdong province, lymphonodi associated with postweaning multisystemic wasting syndrome were collected from pearl river delta in Guangdong province and then the disease materials that proved to be positive by polymerase chain reaction (PCR) method were cultured in PK-15 cells and 5 PCV2 strains were successfully isolated and marked as GD-jm,GD-sz,GD-pz,GD-gj and GD-ss, respectively.The viral genome DNA was extracted and the whole genome of 5 PCV2 isolates were amplified and inserted into pMD18-T Simple Vector. The results were submitted into GenBank and the accession numbers were JX912914,JX912915,JX945575,JX945576 and JX945577.Sequence homology analysis was conducted with the aid of biology software. We found that all of 5 isolates harbored a full length sequence of 1767 bp and 3 of which were designated into PCV2b genotype, the other two were designated into PCV2d genotype.

Key words: porcine circovirus type 2; full length sequence cloning; sequence analysis

CLC Number:

S852.65

TANG Zhi-ling, XIAO Jian-xiong, CHEN Rui-ai, LUO Man-lin. Full Length Sequence Cloning and Sequence Analysis of 5 Porcine Circovirus Type 2 Isolates[J]. , 2013, (5): 14-18.

References

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Full Length Sequence Cloning and Sequence Analysis of 5 Porcine Circovirus Type 2 Isolates

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