脂质体法转染水牛肾脏成纤维细胞条件的优化

Abstract

Abstract: The aim of the present study was to obtain the highest transfection efficiency by liposome induced exogenous DNA to buffalo fetal fibroblasts. In the study, the exogenous DNA was transfected into buffalo fetal fibroblasts using Lipofectamine LTX. The impacts of liposome amount, plasmid amount and size, transfection time course, initial plated density and different promoter used on transfection efficiency were discussed according to the GFP marker gene. It was indicated that 4 μg pmaxGFP plasmid DNA with 4 μL liposome resulted in the highest transfection efficiency and its cell activity was up to 98% after 6 h transfection.

Key words: liposome; fibroblasts; transfection; buffalo

CLC Number:

Q813.1

DENG Ting-xian, PANG Chun-ying, ZHANG Ying-yin, YANG Bing-zhuang, ZHANG Xiu-fang, LIANG Xian-wei. Optimization of the Transfection Efficiency of Buffalo Fetal Fibroblasts Cells Using Liposome[J]. , 2013, (5): 119-122.

[1]	XU Yuanyuan, ZHENG Haiying, YANG Chunyan, DENG Tingxian, HUANG Chenqian, FENG Chao, LU Xingrong, DUAN Anqin, MO Xia, MA Xiaoya, SHANG Jianghua. Effects of Inhibition of BMP/Smad Signaling Pathway on the Growth and Steroid Hormone Synthesis of Buffalo Ovarian Granulosa Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2354-2362.
[2]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[3]	TIAN Shuyue, SHEN Yingchao, WANG Xisheng, WANG Min, YI Minna, Tseweendolmaa, TAO Li, ZHAO Bilig, Manglai, Gerelchimeg. Study on Establishment of Immortalized Cell System of Equine Fetal Fibroblasts in Mongolian Horse [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 46-57.
[4]	LIU Xinyong, TAN Zhengzhun, LI Hui, HUANG Jian, LUO Hua, ZENG Linghu, CHENG Juanru, ZHONG Huapei, YANG Chunyan, WANG Xiaoli, QIN Guangsheng. Effects of Heat Stress on Blood Biochemical Indexes of Murrah and Nili-Ravi Buffaloes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 521-528.
[5]	XU Yuanyuan, MO Xia, HUANG Chenqian, FENG Chao, LU Xingrong, MA Xiaoya, YANG Chunyan, ZHENG Haiying, SHANG Jianghua. Cloning and Bioinformatics Analysis of TRAIL Gene in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3693-3703.
[6]	LIANG Shasha, PANG Chunying, DENG Tingxian, LU Xingrong, DUAN Anqin, MA Xiaoya, FANG Yanyan, LIANG Xianwei. Polymorphism of MFSD2A Gene and Its Association with Milk Production Traits in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3856-3865.
[7]	ZHENG Haiying, YANG Chunyan, LI Lingyu, TANG Liping, ZHENG Wei, SHANG Jianghua. Effects of Resveratrol on Proliferation of Cumulus Cells Cultured in vitro and mRNA Expression Levels of Related Genes in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 208-215.
[8]	LI Lingyu, ZHENG Haiying, CHEN Mingtang, TANG Liping, SHANG Jianghua, YANG Chunyan. Effects of Oocyte Origin on the Developmental Potential of Buffalo Somatic Cell Nuclear Transfer Embryos [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 241-247.
[9]	DUAN Anqin, LU Xingrong, MA Xiaoya, LIANG Shasha, DENG Tingxian. Effect of HSD17B4 Gene Interference on Milk Fat and Casein in Buffalo Mammary Epithelial Cell [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3128-3137.
[10]	MA Xiaoya, LIANG Yanyin, LU Xingrong, DUAN Anqin, LIANG Shasha, DENG Tingxian. Bioinformatics Analysis of ABCD Gene Family in Buffalo and Expression Analysis of ABCD1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3171-3182.
[11]	PANG Chunying, LIANG Shasha, WEN Chongli, CHEN Mingtang, LU Chengwei, WEI Kelong, PAN Yuhong, LIANG Xianwei. Isolation and Identification of Plasma Exosomes in Buffalo Calves [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3200-3205.
[12]	BI Dengfeng, WANG Yu, YAO Jing, ZHAO Jianguo. CRISPR/Cas13d-mediated SUV39H1/SUV39H2 Gene Knockdown in Porcine Embryonic Fibroblasts [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1957-1966.
[13]	YANG Shuai, ZHANG Xinxin, ZHOU Di, YUAN Jing, YANG Liguo. Study on the Growth Promoting Effect of Crossbred Buffalo Growing Cattle Immunized with DNA Vaccine of Co-expression of Somatostatin and Cortistatin [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1361-1369.
[14]	WAN Meng, CHEN Yilin, QIAN Yuhao, HUANG Xuan, XIA Lianchen, WANG Chunwei, ZHU Aixia. Preparation of Cationic Squalene Liposome Adjuvant and Physical and Chemical Properties [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1431-1439.
[15]	ZHANG Huihui, LI Mengwei, TANG Zhenhua, LIANG Xin, PENG Lijuan, PENG Kaiping, YANG Chengjian, WANG Xinfeng. Effects of Cysteamine on the Lactation Performance,Antioxidant Performance and Rumen Microbial Diversity of Buffalo in Summer [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 901-915.

Optimization of the Transfection Efficiency of Buffalo Fetal Fibroblasts Cells Using Liposome

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments