脂质体法转染水牛肾脏成纤维细胞条件的优化

Abstract

Abstract: The aim of the present study was to obtain the highest transfection efficiency by liposome induced exogenous DNA to buffalo fetal fibroblasts. In the study, the exogenous DNA was transfected into buffalo fetal fibroblasts using Lipofectamine LTX. The impacts of liposome amount, plasmid amount and size, transfection time course, initial plated density and different promoter used on transfection efficiency were discussed according to the GFP marker gene. It was indicated that 4 μg pmaxGFP plasmid DNA with 4 μL liposome resulted in the highest transfection efficiency and its cell activity was up to 98% after 6 h transfection.

Key words: liposome; fibroblasts; transfection; buffalo

CLC Number:

Q813.1

DENG Ting-xian, PANG Chun-ying, ZHANG Ying-yin, YANG Bing-zhuang, ZHANG Xiu-fang, LIANG Xian-wei. Optimization of the Transfection Efficiency of Buffalo Fetal Fibroblasts Cells Using Liposome[J]. China Animal Husbandry & Veterinary Medicine, 2013, (5): 119-122.

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Optimization of the Transfection Efficiency of Buffalo Fetal Fibroblasts Cells Using Liposome

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