一株H9N2亚型禽流感病毒分离株的生物学特性研究及其全基因组序列分析

Abstract

Abstract: The biological characteristic and analysis of the genome of a strain H9N2 AIV(A/chicken/Guangdong/QY/2010(H9N2))that isolated from Guangdong province in 2010 was investigated in this paper. The result indicated that EID₅₀ was 10^-9/0.1 mL, MDT was 104 hours, ICPI was 0.51, IVPI was 0; the whole eight cDNA fragments of the virus were amplified by reverse transcription polymerase chain reaction, after cloning and sequencing, gene sequences were analyzed on homology and heredity evolution, the result indicated that HA gene was located on the same branch with Ck/HK/G9/97 and Dk/HK/Y280/97 ,the cleavage site of HA gene was PARSSR↓GLF, 8 potential glycosylation were found on HA gene,the 226 site amino acid was L, the stenoplastic 226 receptor-binding site was changed from L to P, the nucleotide homology of the HA and NA gene to the Dk/HK/Y280/97 was higest, the nucleotide homology of the NS, PA, PB1 gene to the Ck/SH/F/98 was higest,the nucleotide homology of the NP gene to A/VN/1203/04(H5N1)was 95.3%.

Key words: AIV; H9N2 subtype; biological characteristic; clone; whole genome; sequence analysis

CLC Number:

S852.65

LIU Hai-ling, HU Zi-li, LUO Kai-jian. Biological Characteristic and Sequence Analysis of A Strain H9N2 Subtype Avian Influenza Virus[J]. .

References

1 王金良,沈志强,李峰,等. 3株H9N2亚型禽流感病毒HA基因变异分析[J]. 中国家禽,2009,31(10):19~22.

2 甘孟侯.禽流感[M]. 第2版.北京:中国农业出版社,2002.

3 齐岩,袁润余,张贺楠,等. H9N2亚型禽流感病毒广东分离株的全基因克隆及序列分析[J]. 病毒学报,2010,26(3):176~181.

4 李燕,李新生,王泽仁,等. 禽流感病毒CH02株(H9)的鉴定及其NS基因分子特征分析[J]. 中国畜牧兽医,2011,38(4):80~83.

5 陆海融,樊晓辉. 禽流感病毒H9N2的研究进展[J]. 广西医学,2005,27(5):699~701.

6 唐秀英,付朝阳,冯菊艳. H9亚型禽流感的流行与防制[J]. 预防兽医学展,2000,2(4):1~3.

7 郭元吉,李建国,程小雯,等. 禽H9N2亚型流感病毒能感染人的发现[J]. 中华实验和临床病毒学杂志,1999,13(2):105~108.

8 郭元吉,董婕,王敏,等. 甲型流感病毒H9N2亚型毒株血凝素基因特性的研究[J]. 中华医学杂志,2001,114(1):76~79.

9 程宁宁,潘忘明,耿士忠,等. 禽流感病毒A/chicken/Yangzhou/N/2005(H9N2)HA基因的克隆及序列分析[J].中国家禽,2007,29(24):27~29.

10 Brown I H,Banks J,Manvell R J,et al. Recent epidemiology and ecology of influenza A viruses in avian species in Europe and the Middle East[J].Dev Biol(Basel),2006,124:45~50.

11 Butt K M,Smith G J,Chen H,et al. Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003[J].J Clin Microbiol,2005,43(11):5760~5767.

12 Chen B L, Zhang Z J, Chen W B, et al. The rearch of avian influenza virus Ⅰ: isolation and preliminary serological identification of avain influenza virus type A from chicken[J].Chinese Joural of Veterinary Medicine,1994,22(10):3~5.

13 Cong Y L, Wang C F, Yan C M, et al. Swine infection with H9N2 influenza viruses in China in 2004. Virus Gene,2008,36:461~469.

14 Guo Y J, Krauss S, Senne D A, et al. Characterization of the pathogenicity of members of the newly established H9N2 in fluenza virus lineages in Asia[J].Virology, 2000, 267: 279~288.

15 Guan Y, Shortridge K F, Krauss S, et al.H9N2 influenza viruses possessing H5N1 like internal genomes continue to circulate in poultry in southeastern China[J].J Virol, 2000, 74(20):9372~9380.

16 Li C, Yu K, Tian G, et al. Evolution of H9N2 influenza viruses from domestic poultry in Mainland China[J]. Virology,2005,340(1):70~83.

17 Matrosovich M N, Krauss S, Webster R G. H9N2 influenza A viruses from poultry in Asia have human virus like receptor specificity[J]. Virology,2001,281(2): 156~162.

18 Peiris J S, Guan Y, Markwell D, et al. Cocirculation of avian H9N2 and contemporary "human" H3N2 influenza A viruses in pigs in southeastern China:potential for gnetic ressortment[J]. J Virol,2001,75(20):9679~9686.

19 Peiris M, Yuen K Y, Leung C W, et al. Human infection with influenza H9N2[J]. Lancet, 1999, 354(9182):916~917.

20 Subtain S M, Chaudhry Z A, Anjum A A, et al. Study on pathogenesis of low pathogenic avian influenza virus H9 in broiler chickens[J].Pakistan Journal of Zoology,2011,43(5):999~1008.

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Biological Characteristic and Sequence Analysis of A Strain H9N2 Subtype Avian Influenza Virus

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