犬IFN-α优化基因的真核表达及抗病毒活性的研究

Abstract

Abstract: According to the yeast system codon partial tropism, modifying the nucleotide sequence of the mature protein co-don,this research used the Pichia pastorios secretion expression vectors pPICZαC to express canine interferon alpha maturedon,this research used the Pichia pastorios secretion expression vectors pPICZαC to express canine interferon alpha mature protein. CaIFN-α was measured by cytopathic effects with Vero-VSV system,the same methods to measure inhibitory effect on PRV and CDV. The results showed that the canine IFN-α got a high expression in yeast. SDS-PAGE showed the protein got 24 ku, larger than the expected size. The CaIFN-α had species specificity, it had a high antiviral activity to PRV on vero cell, reached 3.6 × 10⁷ U/L, and also had a good inhibition to CDV. Its expression got 58 mg/L. The study provided the base for further study of CaIFN-α activity and its clinical application.

Key words: canine IFN-alpha; optimize gene; yeast expression; antiviral activity

CLC Number:

ZHANG Shu-qiong, GAO Juan, ZENG Si-yao, JIANG Zai-xue, CHEN Rui-ai, LUO Man-lin. The Research of Expression of Optimized Gene of Canine Alpha-Interferon in Pichia pastoris and its Antiviral Activity[J]. .

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The Research of Expression of Optimized Gene of Canine Alpha-Interferon in Pichia pastoris and its Antiviral Activity

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