Abstract: Two monoclonal antibodies against rabies virus glycoprotein were produced by immunization with rabies virus strain BD06 in BALB/c mice. Splenocytes from the immunized mice were fused with SP2/0 mouse myeloma cells. After fusion, antibodies in the supernatant of the hybrid cells were respectively detected by ELISA and FAVN tests. Two hybrid cells secreting antibody binding to rabies virus, i.e. 6F12 and 1B12 strains, were analyzed by Western blotting and FAT methods. Results showed that conformational and linear epitopes of the glycoprotein were repectively recognized by neutralizing monoclonal antibody 6F12 and 1B12.

Key words: rabies virus; glycoprotein; neutralizing antibody; monoclonal antibody