组织型纤溶酶原激活剂基因在牛卵丘-卵母复合体体外成熟进程中的表达

Abstract

Abstract: In the present study,four experiments were conducted to investigate the potential effects of tPA on bovine cumulus and oocytes during in vitro maturation (IVM) (0,8,16,24 h). The expressions of tPA were detected by relative Real-time RT-PCR experiments in bovine cumulus and oocytes both after 0,8,16,24 h of IVM and under different FSH concentrations after 16 h of IVM separately. Meanwhile,the degree of bovine cumulus cell expansion and the percentages of first polar body in oocyte were assessed respectively. TPA mRNA levels were higher in cumulus and oocytes at 16 h and 24 h than those at 0 h and 8 h of IVM significantly (P<0.05). Among those groups that under different FSH concentrations (0,0.01,0.1 and 1 IU/mL) after 16 h of IVM,tPA mRNA levels of cumulus was increased following the elevated FSH concentrations,as well as the cumulus expansion,tPA mRNA of cumulus cells in 1 IU/mL FSH group was significantly higher than others. The results indicated that the expression of tPA in bovine cumulus was regulated positively by FSH,tPA could facilitate the cumulus expansion during IVM. However,it needed to be further investigated that tPA expression might be involved in first polar body excrusion of bovine oocytes.

Key words: tPA; cow; cumulus cells; oocytes; FSH

CLC Number:

S814.8

GUAN Hong-min, MI Yan, ZHANG Ling-li, LI Hai-jun, CAO Gui-fang. Expression of Tissue-type Plasminogen Activator in Bovine Cumulus-oocytes during in vitro Maturation[J]. .

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Expression of Tissue-type Plasminogen Activator in Bovine Cumulus-oocytes during in vitro Maturation

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