Abstract: In order to study the molecular construction of the ISG15 gene from Xijiang Bashibai sheep, RNA was extracted from the lymphocyte, which was isolated from the peripheral blood of the Bashibai sheep. The specific primer designed was amplified by the RT-PCR method. Then cloned the gene order of Bashibai sheep, PCR products that were reclaimed were cloned into pMD18-T vector, and then transformed into DH5α, detected masculine clone, proceeded sequence analysis. The conclusion showed that total length of the genetic coding region cloned from Bashibai sheep is 522 bp, and 172 amino acids were encoded. BLAST analysis showed that Bashibai sheep ISG15 gene shared 99%, 98%, 95%,94%,94% and 82% homology with those of Ovis aries, goat, Ovis aries breed Small Tail Han, Bubalus bubalis, bos and Sus scrofa respectively. Molecular phylogenetic tree analysis showed that Bashibai sheep assembled to Ovis aries, then assembled to Ovis aries breed small Tail Han and bos. The clustering was identical to the biological classification.

Key words: Bashibai sheep; ISG15 gene; clone; sequence analysis