一株鸡传染性法氏囊病病毒的分离鉴定

Abstract

Abstract: One isolate of infectious bursal disease virus (IBDV),was isolated from the vaccination failure chicken. Through serologic AGP experiment,results showed obvious white precipitation line; the high variable (hv) region of VP2 genes were amplified by RT-PCR (reverse transcriptase polymerase chain reaction) and were compared for their sequences. It was indicated that the homology of TJ-hg strain and very virulent IBDV strain all reached above 90% and 98.8% to 99.3% at nucleotide level,respectively. And the change of amino acid was complement in high variable (hv) region of VP2 genes. The mutations amino acid Q219P located in the first hydrophilic region of TJ-hg strain. The research demonstrated that TJ-hg strain belonged to vvIBDV,but it was different from standard strain.

Key words: IBDV; very virulent strain; molecular identification

CLC Number:

S852.65

ZHAO Yun-ying, ZHANG Yan-xin, ZHANG Wen-sheng, LI Fu-gui. Isolation and Identification of One Strain Infectious Bursal Disease Virus[J]. , 2012, 39(6): 207-211.

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Isolation and Identification of One Strain Infectious Bursal Disease Virus

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