Abstract: In order to reveal the effects on ovulation and reproduction traits, differentially expressed genes in pig ovaries was detected. We performed the mRNA differential diplay (DD) technique to investigate the differences in gene expression in the ovary tissues from Meishan and Duroc. Nine expressed sequence tags (ESTs), differentially expressed between Meishan and Duroc, were isolated and identified through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The nucleotide sequence analysis revealed that the 3 ESTs showed similarity to known genes published in GenBank. Tissue expression profile analysis showed that the ESTs were expressed differently in heart, liver, lung, kidney, muscle, fat (subcutaneous fat little legs, the same below), small intestine, brain, ovary, uterus, spleen, oviduct. The results indicated the diversity of differential display direction of genes between Meishan and Duroc. The results also suggested that the differentially expressed genes will resulte in the differences of reproductive traits in pigs and provide the basic research for further study on the molecular mechanism of ovulation regulatory network.

Key words: Meishan; Duroc; ovary; semi-quantitative; expression profiles