IGF-Ⅰ在奶牛乳腺的表达及其对乳腺上皮细胞泌乳功能的影响

Abstract

Abstract: To explore the relationship between expression of IGF-Ⅰand development and lactation ability of dairy cow mammary gland, the expression of IGF-Ⅰ in dairy cow mammary tissue was detected using qRT-PCR. The cell culture, qRT-PCR, and MTT assay were also used to test the effects of IGF-Ⅰ on dairy cow mammary epithelial cells. The results showed that expression of IGF-Ⅰ in mammary tissue in puberty cow was the highest. After IGF-Ⅰ addition, IGF-ⅠR expression, proliferation ability of mammary epithelial cells and beta casein expression were raised. The results suggest that IGF-Ⅰ probably promotes IGF-ⅠR expression, further encourages the proliferation of mammary epithelial cells and increases beta casein content.

Key words: dairy cow; IGF-Ⅰ; IGF-ⅠR; expression; lactation

CLC Number:

Q579.1⁺3

ZHANG Tong-fei;CUI Ying-jun;LI Qing-zhang. Expression of IGF-Ⅰin Mammary Gland and the Effects of IGF-Ⅰ on Mammary Epithelial Cells[J]. , 2012, 39(5): 126-129.

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Expression of IGF-Ⅰin Mammary Gland and the Effects of IGF-Ⅰ on Mammary Epithelial Cells

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