Abstract: To explore the relationship between the influence of proliferation and laminin belong to extracellular matrix in mammary epithelial cell of dairy cow from different hormone and cytokine related lactation. Took the normal mammary glands tissue in lactation dairy cow and cultured the mammary epithelial cells in vitro, deteminate the influence of prolactin (PRL), bovine growth hormone (GH), insulin-like growth factor-1 (IGF-Ⅰ), insulin-like growth factor-2 (IGF-Ⅱ) on the proliferation of mammary epithelial cells in dairy cow with MTT under laminin coating or not. The facilitation in proliferation with blood serum, differernt hormone and cytokine related lactation was found by GH and IGF-Ⅰ (P<0.05) under laminin coating, cell survival was maintained by PRL and IGF-Ⅱ (P<0.05).Under the condition of un-blood serum, different hormone and cytokine related lactation haplo-addition has phanero-less proliferation (P>0.05). Cell growth has different encouraged by PRL, GH, IGF-Ⅰ and IGF-Ⅱ with laminin uncoating and blood serum (P<0.05); the proliferation was higher up-setted by IGF-Ⅰ (P<0.05). Laminin as culture matrix is encourage-less to the normal mammary epithelial cells in lactation dairy cow cultured in vitro, which is beneficial for auxo-survival by PRL and IGF-Ⅱ. The action of proliferation and survival were encouraged by PRL, GH, IGF-Ⅰ and IGF -Ⅱ, which needed blood serum. The proliferation ability of IGF-Ⅰ is highest.

Key words: prolactin; bovine growth hormone; insulin-like growth factor-1; insulin-like growth factor-2; laminin; mammary epithelial cells of dairy cow