Abstract: For further studies in canine nuclear transfer, the culture and purification methods of canine ear and abdominal skin fibroblast cell were studied. The results showed the cells could be seen around ear tissue after 4 d culture and quick growth were seen about 8 to 10 d, after 2 weeks the bottle could be covered with cells. In abdominal skin group, cells could be seen after about 6 to 7 d and lots of cells around tissue appeared after about 10 d. The bottom of cell bottle would fully cover with cells after 20 d in abdominal skin group.The growth speed of abdominal skin fibroblast cells were lower than ear fibroblast cells in early stages of the cell lines establishment, but when cells grew to 4 passages, both the two cell lines had the same growth curve.

Key words: canine; fibroblast; growth curve