China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (11): 4768-4775.doi: 10.16431/j.cnki.1671-7236.2023.11.043

• Basic Veterinary Medicine • Previous Articles     Next Articles

Therapeutic Clinical Effect of Canine UC-MSCs Combined with Trochlear Groove Reconstruction on Patellar Dislocation in Dogs

HE Shi1, XIAN Weihang1, WU Zhongheng2, CHEN Shengfeng1, RUAN Huimin3, YE Cailing3, WANG Cuilin3, WANG Bingyun1   

  1. 1. School of Life Science and Engineering, Foshan University, Foshan 528231, China;
    2. Doctor Veterinary Hospital of Guangzhou, Guangzhou 510000, China;
    3. Guangdong VetCell Biological Technology Co., Ltd., Foshan 528225, China
  • Received:2023-04-08 Online:2023-11-05 Published:2023-10-27

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Abstract: 【Obgective】 The aim of this study was to investigate the therapeutic clinical effect of canine umbilical cord mesenchymal stem cells (UC-MSCs) combined with trochlear groove reconstruction in the treatment of patellar dislocation.【Method】 Twenty dogs with patellar dislocation were randomly divided into stem cell treatment group and conventional surgery group, with 10 dogs in each group. On the day of surgery, 0.5 mL UC-MSCs (106/kg) suspension was injected into the joint cavity of the stem cell treatment group, and the same amount of normal saline was injected into the conventional surgery group.The therapeutic effect of UC-MSCs was evaluated by collecting the basic information of the affected dogs, follow-up records, blood routine and blood factor content detection, and imaging methods. 【Result】 Compared with the conventional surgery group, the interleukin-6 (IL-6) content in the stem cell treatment group was extremely significantly or significantly decreased at the 1st and 7th day after surgery (P<0.01 or P<0.05).There were no significant differences in transforming growth factor-β1 (TGF-β1), matrix metalloproteinase 13 (MMP-13), tumor necrosis factor α (TNF-α), total number of white blood cells and neutrophils between two groups (P>0.05). Upon reassessment 30 days postoperatively via digital X-ray, the stem cell treatment exhibited pronounced cartilage and bone regeneration at the bone lesion location, with no complications such as recurrence and arthritis observed.In the conventional surgery group, the bone growth at the bone injury site was slow, and 2 patients suffered from re-dislocation of patella (2/10).【Conclusion】 After trochlear groove reconstruction, intraarticular injection of UC-MSCs could promote cartilage and bone tissue growth and reduce the risk of recurrence under conventional post-operative care (anti-inflammatory, antibacterial, and pain reliever).UC-MSCs combined with trochlear groove reconstruction could improve the clinical treatment of patellar dislocation in dogs.

Key words: canine umbilical cord mesenchymal stem cells; trochlear groove reconstruction; patellar dislocation; bone repair

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